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Journal of Cell Science, Vol 92, Issue 3 433-439, Copyright © 1989 by Company of Biologists

JOURNAL ARTICLES

Identification and localization of major cortical proteins in the ciliated protozoan, Euplotes eurystomus

NE Williams, JE Honts, Q Lu, CL Olson and KC Moore
Department of Biology, University of Iowa, Iowa City 52242.

Shape-preserving cortical residues have been isolated from Euplotes eurystomus cells by the application of Triton X-100 at high ionic strength. These integrated structures consist of articulated plates and widely interspersed cages that formerly contained basal bodies associated with the clusters of cilia characteristic of this cell type. Using SDS-PAGE and immunolocalization procedures, we have identified major subunit proteins of both the plates (116, 110 (X10(3] Mr, and the basal body cages (86 X 10(3) Mr). The potential for studies of these proteins in contributing to our understanding of cortical development and evolution in Euplotes is discussed.


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© The Company of Biologists Ltd 1989