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Journal of Cell Science, Vol 90, 465-473, Copyright © 1988 by Company of Biologists

Submitted on October 22, 1987
Accepted on March 15, 1988

The role of intracellular Ca2+ during early sexual development in Dictyostelium discoideum: effects of LaCl3, LaCl3, Ins(1,4,5)P3, TMB-8, chlortetracycline and A23187 on cell fusion

MICHAEL A. LYDAN 1 and DANTON H. O'DAY 1

1 Department of Biology, University of Toronto, Erindale Campus, Mississauga, Ontario, Canada L5L 1C6

Author for correspondence

The agents LaCl3, Ins(1,4,5)P3, TMB-8, chlortetracycline (CTC) and A23187 were used to study the requirement for internal calcium mobilization during gamete cell fusion in Dictyostelium discoideum. The inhibition of the influx of calcium (LaCl3) prevented cell fusion in a dose-dependent manner. At the intracellular level, Ins(1,4,5)P3, an endogenous regulator of calcium release from intracellular stores, stimulated cell fusion within one hour following its addition. Treatment with agents that prevent the release of calcium from intracellular stores (TMB-8, CTC) also inhibited cell fusion in a dose-dependent manner. However, the non-specific augmentation of cytosolic calcium levels through the use of the ionophore A23187 inhibited cell fusion, and the amount inhibition was directly related to the drug concentration. Studies on cell morphology and growth plus results from reversibility experiments involving the ability to form macrocysts reveal that these effects are not due to non-specific drug toxicity. In total, these results suggest that the mobilization of calcium both from the extracellular environment and from intracellular stores important and is probably regulated during gamete cell fusion in D. discoideum.

Key words: calcium, cell fusion, calcium antagonists, Dictyostelium

Submitted on October 22, 1987
Accepted on March 15, 1988




This article has been cited by other articles:


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M. Lydan and D. Cotter
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J. Cell Sci., January 5, 1995; 108(5): 1921 - 1930.
[Abstract] [PDF]


© The Company of Biologists Ltd 1988