Journal of Cell Science, Vol 9, 453-473, Copyright © 1971 by Company of Biologists

Submitted on December 23, 1970

Ultrastructure of the Fungal Nucleus

I. The Kinetochore Equivalent (KCE)

¹ Zentralinstitut für Mikrobiologie und experimentelle Therapie der Deutschen Akademie der Wissenschaften zu Berlin 69 Jena, DDR

The electron-dense material which regularly occurs at the poles of the nuclei of certain fungi during division has been studied in hyphal interphase nuclei of 4 basidio- and 3 ascomycetes. The shape of this material varies with the species and the nuclear age and may be that of a sphere or a dumbbell. In some instances paired diskoidal structures are found in the place of the globular elements of the dumbbell configuration. The organelle in question lies in an invagination of the nuclear envelope preferentially at the forward pointing pole of the nucleus. Its size (0.1-0.5 µm) is correlated with the size of the nucleus. In Polystictus versicolor the diameter of both globular elements of the polar organelle increases during initiation of the division process.

The structure is identical with the fungal ‘centriole’ of light-microscopical studies but lacks the characteristic organization of a true centriole. It is suggested that the polar organelle is the equivalent of a kinetochore because it maintains continuous and direct contact with the nuclear envelope (on its cytoplasmic aspect), seems to be connected with the karyoplasm, exhibits oscillating movements, is continuous with microtubules during division and resembles in its fine structure the kinetochores of the chromosomes of higher plants and animals. In addition to its function as an organelle of nuclear motility it also seems to play a part in the initiation of DNA synthesis and nuclear division. If this is correct one would expect to find the genome of the fungal nucleus having the form of a unitary, coherent compound structure (chain) extending from or incorporating a single, solitary kinetochore.