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Journal of Cell Science, Vol 88, Issue 2 145-149, Copyright © 1987 by Company of Biologists

JOURNAL ARTICLES

Laser-induced fusion of mammalian cells and plant protoplasts

R Wiegand, G Weber, K Zimmermann, S Monajembashi, J Wolfrum and KO Greulich
Physikalisch-Chemisches Institut, Ruprecht-Karls-Universitat, Heidelberg, FRG.

An ultraviolet-laser microbeam was shown to be suitable for inducing fusion of individually selected plant protoplasts or of B-lymphocytes with myeloma cells. The fusion took place in normal culture medium and the fusogenic condition perturbed the cells only for a fraction of a millisecond. Without manipulating the cell culture except for exposing the cells to laser light, fusion products between preselected individual pairs may be produced.


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A Kurkdjian, G Leitz, P Manigault, A Harim, and K. Greulich
Non-enzymatic access to the plasma membrane of Medicago root hairs by laser microsurgery
J. Cell Sci., January 5, 1993; 105(1): 263 - 268.
[Abstract] [PDF]


© The Company of Biologists Ltd 1987