spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Aerts, R. J.
Right arrow Articles by Konijn, T. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Aerts, R. J.
Right arrow Articles by Konijn, T. M.

Journal of Cell Science, Vol 87, Issue 3 423-430, Copyright © 1987 by Company of Biologists

JOURNAL ARTICLES

Cytoplasmic pH at the onset of development in Dictyostelium

RJ Aerts, AJ Durston and TM Konijn
Cell Biology and Morphogenesis Unit, University of Leiden, The Netherlands.

Cytoplasmic pH (pHi) was measured by a pH 'null point' method in synchronous populations of Dictyostelium discoideum cells. Synchronous populations of vegetative cells with a lower pHi than asynchronous populations of vegetative cells show a clear preference for the prespore differentiation pathway when they enter development. Treatment of these cells before development with the weak base ammonia results in an increase in pHi and an abolition of the preference of the cells for the prespore pathway. On the other hand, synchronous populations of vegetative cells with the same pHi as asynchronous populations of vegetative cells resemble these last cells in that they show no preference for a certain differentiation direction when they enter development. However, both these synchronous and asynchronous populations of cells do acquire a preference for the prespore pathway when they are treated before development with the weak acid 5,5-dimethyl-2,4-oxazolidineolione (DMO), which lowers their pHi. It is concluded that changes in pHi at the onset of the developmental program lead to significant changes in the preference of the cells for the prespore differentiation pathway in Dictyostelium discoideum.


This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
M Stoeckelhuber, A. Noegel, C Eckerskorn, J Kohler, D Rieger, and M Schleicher
Structure/function studies on the pH-dependent actin-binding protein hisactophilin in Dictyostelium mutants
J. Cell Sci., January 7, 1996; 109(7): 1825 - 1835.
[Abstract] [PDF]

Home page
 J. Biol. Chem.Home page
D. T. Brazill, D. R. Caprette, H. A. Myler, R. D. Hatton, R. R. Ammann, D. F. Lindsey, D. A. Brock, and R. H. Gomer
A Protein Containing a Serine-rich Domain with Vesicle Fusing Properties Mediates Cell Cycle-dependent Cytosolic pH Regulation
J. Biol. Chem., June 16, 2000; 275(25): 19231 - 19240.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 1987