|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 83, Issue 1 235-249, Copyright © 1986 by Company of Biologists
JOURNAL ARTICLES |
J Fantini, B Abadie, A Tirard, L Remy, JP Ripert, A el Battari and J Marvaldi
The replacement of glucose by galactose in the culture medium resulted in partial structural and functional enterocytic differentiation of HT29 cells. In order to characterize populations of homogeneously differentiated HT29 cells we have selected two clonal cell lines HT29-D4 and HT29-D9 with the following functional and structural characteristics when grown in a galactose-containing medium: the two clonal cell populations were permanently morphologically differentiated as shown by the presence of mature junctional complexes and a well-organized brush border (especially for HT29-D4 cells); HT29-D4 and HT29-D9 cells were able to form domes early in confluency, which indicated a functional state of differentiation; the process of differentiation was fully reversible when glucose was added to the culture medium. The induction of domes was investigated in these two cell populations and we demonstrated for the first time that proteolytic enzymes are potent inducers of dome formation. The architecture of domes either obtained spontaneously or induced by proteolytic enzymes was not maintained in the presence of ouabain (a specific inhibitor of the Na+/K+-ATPase). In conclusion, HT29-D4 and HT29-D9 cells can be maintained permanently in a differentiated state in a glucose-free medium and were able to form domes at confluency. The observation that proteolytic enzymes were able to induce dome formation can help in the comprehension of the mechanism involved in the establishment of the differentiated state.
This article has been cited by other articles:
|
|
 |
|
  P. Rouet-Benzineb, C. Rouyer-Fessard, A. Jarry, V. Avondo, C. Pouzet, M. Yanagisawa, C. Laboisse, M. Laburthe, and T. Voisin Orexins Acting at Native OX1 Receptor in Colon Cancer and Neuroblastoma Cells or at Recombinant OX1 Receptor Suppress Cell Growth by Inducing Apoptosis J. Biol. Chem., October 29, 2004; 279(44): 45875 - 45886. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Darmoul, V. Gratio, H. Devaud, T. Lehy, and M. Laburthe Aberrant Expression and Activation of the Thrombin Receptor Protease-Activated Receptor-1 Induces Cell Proliferation and Motility in Human Colon Cancer Cells Am. J. Pathol., May 1, 2003; 162(5): 1503 - 1513. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Maresca, R. Mahfoud, N. Garmy, and J. Fantini The Mycotoxin Deoxynivalenol Affects Nutrient Absorption in Human Intestinal Epithelial Cells J. Nutr., September 1, 2002; 132(9): 2723 - 2731. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Lehmann, F. Andre, C. Bellan, M. Remacle-Bonnet, F. Garrouste, F. Parat, J.-C. Lissitsky, J. Marvaldi, and G. Pommier Deficient Processing and Activity of Type I Insulin-Like Growth Factor Receptor in the Furin-Deficient LoVo-C5 Cells Endocrinology, September 1, 1998; 139(9): 3763 - 3771. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Bernard-Perrone, J. Carrere, W. Renaud, C. Moriscot, K. Thoreux, P. Bernard, A. Servin, D. Balas, and F. Senegas-Balas Pancreatic trypsinogen I expression during cell growth and differentiation of two human colon carcinoma cells Am J Physiol Gastrointest Liver Physiol, June 1, 1998; 274(6): G1077 - G1086. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V Rigot, M Lehmann, F Andre, N Daemi, J Marvaldi, and J Luis Integrin ligation and PKC activation are required for migration of colon carcinoma cells J. Cell Sci., January 10, 1998; 111(20): 3119 - 3127. [Abstract] [PDF]
|
|
|
|
|
|
J. A. Hochman, D. Sciaky, T. L. Whitaker, J. A. Hawkins, and M. B. Cohen Hepatocyte nuclear factor-1alpha regulates transcription of the guanylin gene Am J Physiol Gastrointest Liver Physiol, October 1, 1997; 273(4): G833 - G841. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. L. Garrouste, M. M. Remacle-Bonnet, M. M.-A. Lehmann, J. L. Marvaldi, and G. J. Pommier Up-Regulation of Insulin/Insulin-Like Growth Factor-I Hybrid Receptors during Differentiation of HT29-D4 Human Colonic Carcinoma Cells Endocrinology, May 1, 1997; 138(5): 2021 - 2032. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Delézay, S. Baghdiguian, and J. Fantini The Development of Na[IMAGE]-dependent Glucose Transport during Differentiation of an Intestinal Epithelial Cell Clone Is Regulated by Protein Kinase C J. Biol. Chem., May 26, 1995; 270(21): 12536 - 12541. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F Nicolas, M. Tiveron, J Davoust, and H Reggio GPI membrane anchor is determinant in intracellular accumulation of apical plasma membrane proteins in the non-polarized human colon cancer cell line HT-29 18 J. Cell Sci., January 10, 1994; 107(10): 2679 - 2689. [Abstract] [PDF]
|
|
|
|
 |
|
 D H van Weering, P D Baas, and J L Bos A PCR-based method for the analysis of human CD44 splice products. Genome Res., October 1, 1993; 3(2): 100 - 106. [Abstract] [PDF]
|
|
