|
|
|
|
|
||
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 80, Issue 1 253-268, Copyright © 1986 by Company of Biologists
JOURNAL ARTICLES |
K Tanaka and T Kanbe
Nuclear division in Schizosaccharomyces pombe has been studied in transmission electron micrographs of sections of cells fixed by a method of freeze-substitution. We have found cytoplasmic microtubules in the vicinity of the spindle pole bodies and two kinds of microtubules, short discontinuous ones and long, parallel ones in the intranuclear mitotic spindle. For most of the time taken by nuclear division the spindle pole bodies face each other squarely across the nuclear space but early in mitosis they briefly appear twisted out of alignment with each other, thereby imparting a sigmoidal shape to the bundle of spindle microtubules extending between them. This configuration is interpreted as indicating active participation of the spindle in the initial elongation of the dividing nucleus. It is proposed that mitosis is accompanied by the shortening of chromosomal microtubules simultaneously with the elongation of the central pole-to-pole bundle of microtubules of the intranuclear spindle. Daughter nuclei are separated by the sliding apart of interdigitating microtubules of the spindle at telophase. Some of the latter bear dense knobs at their ends.
This article has been cited by other articles:
|
|
 |
|
  A. Franco, J. C. Meadows, and J. B. A. Millar The Dam1/DASH complex is required for the retrieval of unclustered kinetochores in fission yeast J. Cell Sci., October 1, 2007; 120(19): 3345 - 3351. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. J. Celio, M. Padamsee, B. T.M. Dentinger, K. A. Josephsen, T. S. Jenkinson, E. G. McLaughlin, and D. J. McLaughlin Septal pore apparatus and nuclear division of Auriscalpium vulgare. Mycologia, September 1, 2007; 99(5): 644 - 654. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Uzawa, F. Li, Y. Jin, K. L. McDonald, M. B. Braunfeld, D. A. Agard, and W. Z. Cande Spindle Pole Body Duplication in Fission Yeast Occurs at the G1/S Boundary but Maturation Is Blocked until Exit from S by an Event Downstream of Cdc10+ Mol. Biol. Cell, December 1, 2004; 15(12): 5219 - 5230. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Sagolla, S. Uzawa, and W. Z. Cande Individual microtubule dynamics contribute to the function of mitotic and cytoplasmic arrays in fission yeast J. Cell Sci., December 15, 2003; 116(24): 4891 - 4903. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. S. Salus, J. Demeter, and S. Sazer The Ran GTPase System in Fission Yeast Affects Microtubules and Cytokinesis in Cells That Are Competent for Nucleocytoplasmic Protein Transport Mol. Cell. Biol., December 15, 2002; 22(24): 8491 - 8505. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Heitz, J. Petersen, S. Valovin, and I. M. Hagan MTOC formation during mitotic exit in fission yeast J. Cell Sci., March 14, 2002; 114(24): 4521 - 4532. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Pidoux, S Uzawa, P. Perry, W. Cande, and R. Allshire Live analysis of lagging chromosomes during anaphase and their effect on spindle elongation rate in fission yeast J. Cell Sci., January 12, 2000; 113(23): 4177 - 4191. [Abstract] [PDF]
|
|
|
|
|
|
K. Nabeshima, T. Nakagawa, A. F. Straight, A. Murray, Y. Chikashige, Y. M. Yamashita, Y. Hiraoka, and M. Yanagida Dynamics of Centromeres during Metaphase-Anaphase Transition in Fission Yeast: Dis1 Is Implicated in Force Balance in Metaphase Bipolar Spindle Mol. Biol. Cell, November 1, 1998; 9(11): 3211 - 3225. [Abstract] [Full Text]
|
|
|
|
|
|
R. R. West, E. V. Vaisberg, R. Ding, P. Nurse, and J. R. McIntosh cut11+: A Gene Required for Cell Cycle-dependent Spindle Pole Body Anchoring in the Nuclear Envelope and Bipolar Spindle Formation in Schizosaccharomyces pombe Mol. Biol. Cell, October 1, 1998; 9(10): 2839 - 2855. [Abstract] [Full Text]
|
|
|
|
|
|
P. Radcliffe, D. Hirata, D. Childs, L. Vardy, and T. Toda Identification of Novel Temperature-sensitive Lethal Alleles in Essential beta -Tubulin and Nonessential alpha 2-Tubulin Genes as Fission Yeast Polarity Mutants Mol. Biol. Cell, July 1, 1998; 9(7): 1757 - 1771. [Abstract] [Full Text]
|
|
|
|
 |
|
 A. J. Bridge, M. Morphew, R. Bartlett, and I. M. Hagan The fission yeast SPB component Cut12 links bipolar spindle formation to mitotic control Genes & Dev., April 1, 1998; 12(7): 927 - 942. [Abstract] [Full Text]
|
|
|
|
|
|
I. Hagan The fission yeast microtubule cytoskeleton J. Cell Sci., January 6, 1998; 111(12): 1603 - 1612. [Abstract] [PDF]
|
|
|
|
 |
|
 J. D. Beinhauer, I. M. Hagan, J. H. Hegemann, and U. Fleig Mal3, the Fission Yeast Homologue of the Human APC-interacting Protein EB-1 Is Required for Microtubule Integrity and the Maintenance of Cell Form J. Cell Biol., November 3, 1997; 139(3): 717 - 728. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Moser, M. Flory, and T. Davis Calmodulin localizes to the spindle pole body of Schizosaccharomyces pombe and performs an essential function in chromosome segregation J. Cell Sci., January 8, 1997; 110(15): 1805 - 1812. [Abstract] [PDF]
|
|
|
|
|
|
I Hagan and M Yanagida Evidence for cell cycle-specific, spindle pole body-mediated, nuclear positioning in the fission yeast Schizosaccharomyces pombe J. Cell Sci., January 8, 1997; 110(16): 1851 - 1866. [Abstract] [PDF]
|
|
|
|
|
|
S. Su, Y Tanaka, I Samejima, K Tanaka, and M Yanagida A nitrogen starvation-induced dormant G0 state in fission yeast: the establishment from uncommitted G1 state and its delay for return to proliferation J. Cell Sci., January 6, 1996; 109(6): 1347 - 1357. [Abstract] [PDF]
|
|
|
|
|
|
H Masuda and T Shibata Role of gamma-tubulin in mitosis-specific microtubule nucleation from the Schizosaccharomyces pombe spindle pole body J. Cell Sci., January 1, 1996; 109(1): 165 - 177. [Abstract] [PDF]
|
|
|
|
|
|
K Nabeshima, H Kurooka, M Takeuchi, K Kinoshita, Y Nakaseko, and M Yanagida p93dis1, which is required for sister chromatid separation, is a novel microtubule and spindle pole body-associating protein phosphorylated at the Cdc2 target sites. Genes & Dev., July 1, 1995; 9(13): 1572 - 1585. [Abstract] [PDF]
|
|
|
|
 |
|
 C.E. Alfa, I.M. Gallagher, and J.S. Hyams Subcellular Localization of the p34cdc2/p63cdc13 Protein Kinase in Fission Yeast Cold Spring Harb Symp Quant Biol, January 1, 1991; 56(0): 489 - 494. [Abstract] [PDF]
|
|
|
|
|
|
M.P. Rout and J.V. Kilmartin Yeast Spindle Pole Body Components Cold Spring Harb Symp Quant Biol, January 1, 1991; 56(0): 687 - 692. [Abstract] [PDF]
|
|
