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Journal of Cell Science, Vol 8, 303-315, Copyright © 1971 by Company of Biologists

Submitted on June 29, 1970

Fine Structure Study of Pollen Development in Haemanthus katherinae Baker

II. Microtubules and Elongation of the Generative Cells

JEAN M. SANGER 1 and W. T. JACKSON 1

1 Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire 03755, U.S.A.

The newly formed generative cell of the pollen grain of the African blood lily is spheroidal after its detachment from the pollen wall. Plastids are almost always excluded from the generative cell, while dictyosomes, mitochondria, ribosomes, endoplasmic reticulum, and microtubules are present throughout development of the cell. During the time that the generative cell is spheroidal, microtubules are found scattered randomly throughout the cytoplasm. The cell subsequently elongates and concurrently an oriented system of microtubules appears along the wall of the cell. The microtubules are aligned with their long axes parallel to the long axis of the cell. This system of microtubules persists in the generative cell throughout its development. The microtubules can be destroyed by exposure to isopropyl N-phenylcarbamate or colchicine and as a result the generative cell reverts to a near spheroidal shape.

Submitted on June 29, 1970







© The Company of Biologists Ltd 1971