spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Shields, J. M.
Right arrow Articles by Haston, W. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shields, J. M.
Right arrow Articles by Haston, W. S.

Journal of Cell Science, Vol 74, Issue 1 75-93, Copyright © 1985 by Company of Biologists

JOURNAL ARTICLES

Behaviour of neutrophil leucocytes in uniform concentrations of chemotactic factors: contraction waves, cell polarity and persistence

JM Shields and WS Haston

The essential component of any hypothesis of random or directed cell movement is the mechanism of cell polarity. In this paper we describe the polar behaviour of human neutrophil leucocytes in uniform concentrations of chemotactic factors both in suspension and while moving across surfaces. Neutrophils exposed to uniform concentrations of chemotactic factors in suspension around the dissociation constant (Kd) for the receptor rapidly become distinctly bipolar; neutrophils exposed to supraoptimal uniform concentrations (100-fold greater than Kd) of chemotactic factors in suspension, although morphologically active, never reached the same degree of polarity as cells in optimal concentrations. These differences in polarity were shown to be the direct result of equatorial contraction waves stimulated on the cell surface by interaction with chemotactic factors. In optimal concentrations of chemotactic factors, contraction waves were initiated from one region of the cell, whereas in supraoptimal concentrations of chemotactic factors contraction waves emanated from all areas of the cell surface. Asymmetry in the distribution of surface receptors for Fc and C3b were observed in neutrophils polarized in uniform concentrations of chemotactic factor. In neutrophils, motile but not well polarized (in 10(-6) M-N-formylmethionyl-leucyl-phenylalanine (fMLP), receptors were uniformly distributed. In neutrophils polarized in concentrations of fMLP near the Kd for the receptor (10(-8) M) receptors for C3b and Fc were localized in the anterior region of the moving cell. The link between contraction waves, cell polarity and receptor redistribution and their initiation by chemotactic peptides is discussed in the context of neutrophil locomotion and response to chemical signals.


This article has been cited by other articles:


Home page
 JCBHome page
K. Wong, A. Van Keymeulen, and H. R. Bourne
PDZRhoGEF and myosin II localize RhoA activity to the back of polarizing neutrophil-like cells
J. Cell Biol., December 17, 2007; 179(6): 1141 - 1148.
[Abstract] [Full Text] [PDF]

Home page
 GENES CELLSHome page
Y. Takai, K. Kitano, S.-i. Terawaki, R. Maesaki, and T. Hakoshima
Structural basis of PSGL-1 binding to ERM proteins.
Genes Cells, December 1, 2007; 12(12): 1329 - 1338.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
K. Wong, O. Pertz, K. Hahn, and H. Bourne
Neutrophil polarization: Spatiotemporal dynamics of RhoA activity support a self-organizing mechanism.
PNAS, March 7, 2006; 103(10): 3639 - 3644.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
J. L. Alonso-Lebrero, J. M. Serrador, C. Dominguez-Jimenez, O. Barreiro, A. Luque, M. A. del Pozo, K. Snapp, G. Kansas, R. Schwartz-Albiez, H. Furthmayr, et al.
Polarization and interaction of adhesion molecules P-selectin glycoprotein ligand 1 and intercellular adhesion molecule 3 with moesin and ezrin in myeloid cells
Blood, April 1, 2000; 95(7): 2413 - 2419.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
E. F. Foxman, E. J. Kunkel, and E. C. Butcher
Integrating Conflicting Chemotactic Signals: The Role of Memory in Leukocyte Navigation
J. Cell Biol., November 1, 1999; 147(3): 577 - 588.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
S. Webb, J. Pollard, and G. Jones
Direct observation and quantification of macrophage chemoattraction to the growth factor CSF-1
J. Cell Sci., January 4, 1996; 109(4): 793 - 803.
[Abstract] [PDF]

Home page
 J. Cell Sci.Home page
H Lepidi, Y Zaffran, J. Ansaldi, J. Mege, and C Capo
Morphological polarization of human polymorphonuclear leucocytes in response to three different chemoattractants: an effector response independent of calcium rise and tyrosine kinases
J. Cell Sci., January 4, 1995; 108(4): 1771 - 1778.
[Abstract] [PDF]

Home page
 J. Cell Sci.Home page
Y Zaffran, H Lepidi, P Bongrand, J. Mege, and C Capo
F-actin content and spatial distribution in resting and chemoattractant-stimulated human polymorphonuclear leucocytes. Which role for intracellular free calcium?
J. Cell Sci., January 7, 1993; 105(3): 675 - 684.
[Abstract] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
D. R. Ambruso, C. Knall, A. N. Abell, J. Panepinto, A. Kurkchubasche, G. Thurman, C. Gonzalez-Aller, A. Hiester, M. deBoer, R. J. Harbeck, et al.
Human neutrophil immunodeficiency syndrome is associated with an inhibitory Rac2 mutation
PNAS, April 25, 2000; 97(9): 4654 - 4659.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 1985