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Journal of Cell Science, Vol 7, 5-13, Copyright © 1970 by Company of Biologists

Submitted on December 3, 1969

The Concentrations of Water, Sodium and Potassium in the Nucleus and Cytoplasm of Amphibian Oocytes

T. J. CENTURY 1, I. R. FENICHEL 2, and S. B. HOROWITZ 2

1 Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, U.S.A.
2 Laboratory of Cellular Biophysics, Albert Einstein Medical Center, Philadelphia, Pennsylvania 19141, U.S A.

Techniques are described for the isolation of nuclei and cytoplasm from frozen amphibian oocytes at -40 °C and their analysis for water, sodium, and potassium contents. Nuclei of the 4 species studied contain 74-85% water while cytoplasm is only 35-50% water. Nuclei contain 7-17 µequiv. Na/ml H2O and 123-129 µequiv. K/ml H2O; cytoplasm contains 59-84 µequiv. Na/ml H2O and 81-94 µequiv. K/ml H2O. Mechanisms suggested to explain the observed nucleo-cytoplasmic asymmetries are the selective sequestering of sodium in the cytoplasm and a fraction of cytoplasmic water which does not act as solvent. Asymmetric cation transport by the nuclear membrane is not indicated.

Submitted on December 3, 1969




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© The Company of Biologists Ltd 1970