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Journal of Cell Science, Vol 7, 15-33, Copyright © 1970 by Company of Biologists

Submitted on November 19, 1969

The Haemoglobin in the Condensed Chromatin of Mature Amphibian Erythrocytes: A Further Study

J. V. SMALL 1 and H. G. DAVIES 2

1 Medical Research Council Biophysics Unit, Department of Biophysics, King's College, 26-29 Drury Lane, London, W.C.2, England; Institute of Biophysics, Aarhus University, Aarhus 8000, Denmark
2 Medical Research Council Biophysics Unit, Department of Biophysics, King's College, 26-29 Drury Lane, London, W.C.2, England

The condensed chromatin in mature nucleated erythrocytes is surrounded by nuclear haemoglobin at a concentration about equal to that in the cytoplasm. New measurements employing improved fixation, glutaraldehyde instead of OsO4, and microspectrophotometric analyses instead of photographic photometry at one wavelength, show that previously reported values for the content of haemoglobin in the mass of the chromatin in Triturus cristatus, 45-50%, were far too high. In the condensed chromatin of 3 species of amphibia the concentrations of intrachromatin haemoglobin relative to the cytoplasm are 10% (Triturus cristatus), 10% (Necturus maculosa) and 8% (Amphiuma tridactylum). It is concluded that haemoglobin is only a minor component of chromatin, and this result is discussed in relation to the condensation of erythrocyte chromatin and observations on its ultrastructure.

Submitted on November 19, 1969






© The Company of Biologists Ltd 1970