spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by White, M. K.
Right arrow Articles by Harris, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by White, M. K.
Right arrow Articles by Harris, H.

Journal of Cell Science, Vol 62, Issue 1 49-80, Copyright © 1983 by Company of Biologists

JOURNAL ARTICLES

Kinetic parameters of hexose transport in hybrids between malignant and nonmalignant cells

MK White, ME Bramwell and H Harris

Matched pairs of isogeneic hybrid cells, in which one member of the pair was malignant and the other not, were used to examine the linkage between malignancy and functional alterations in hexose transport. The kinetic parameters of uptake of 2-deoxy-D-glucose were measured in a range of such hybrids, both human and murine. Some other malignant cell lines were also examined and were compared with non-tumorigenic derivatives of tumour cells selected by exposure to the lectin, wheat-germ agglutinin. In every case, malignancy, as defined by the ability of cells to grow progressively in vivo, was found to be linked to a decrease in the Michaelis constant of hexose uptake. Independent measurement of the transport and phosphorylation reactions involved in hexose uptake revealed that this decrease was determined by the membrane transport system. The difference in Michaelis constant between malignant and non-malignant cells was observed with 3-O-methylglucose, a hexose that is transported into the cell but not further metabolized. The activity of hexokinase in cell homogenates was higher than the level that would be required to cope with transport and showed no correlation with tumorigenicity. Measurement of the uptake of D-glucose itself, by a rapid filtration centrifugation method, gave results similar to those obtained with 2-deoxy-D-glucose.


This article has been cited by other articles:


Home page
 BloodHome page
A. S. Tan, N. Ahmed, and M. V. Berridge
Acute Regulation of Glucose Transport After Activation of Human Peripheral Blood Neutrophils by Phorbol Myristate Acetate, fMLP, and Granulocyte-Macrophage Colony-Stimulating Factor
Blood, January 15, 1998; 91(2): 649 - 655.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
T Kitagawa, Y Tsuruhara, M Hayashi, T Endo, and E. Stanbridge
A tumor-associated glycosylation change in the glucose transporter GLUT1 controlled by tumor suppressor function in human cell hybrids
J. Cell Sci., January 12, 1995; 108(12): 3735 - 3743.
[Abstract] [PDF]


© The Company of Biologists Ltd 1983