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Journal of Cell Science, Vol 61, Issue 1 289-297, Copyright © 1983 by Company of Biologists

JOURNAL ARTICLES

Adaptation of human diploid fibroblasts in vitro to serum from different sources

GB Zamansky, C Arundel, H Nagasawa and JB Little

The growth of two human diploid skin fibroblast cell lines, originally grown in medium supplemented with foetal bovine serum and later adapted to medium supplemented with newborn bovine, bovine calf or horse serum, has been studied. Prolonged generation times increased cell volumes and decreased plating efficiencies were observed in cultures grown in newborn bovine, bovine calf or horse serum. In general, the deleterious effects were most severe as a result of growth in bovine calf or horse serum. In the light of the present findings, we believe investigators should exert great caution in switching human fibroblast cultures from foetal bovine serum to alternative sera, even at times of scarcity and high prices.




© The Company of Biologists Ltd 1983