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Journal of Cell Science, Vol 58, Issue 1 35-48, Copyright © 1982 by Company of Biologists

JOURNAL ARTICLES

Gene expression in Acetabularia. II. Analysis of in vitro translation products

RL Shoeman and HG Schweiger

The translation products induced by poly(A)+ RNA from Acetabularia mediterranea, A. cliftonii and A. ryukyuensis in a modified, highly efficient wheat germ cell-free in vitro system were separated by two-dimensional gel electrophoresis. A comparison of the translation products on the basis of their molecular weight and their isoelectric point revealed only a limited similarity between the patterns of the three species. The pronounced species specificity will permit the study of the in vivo translation of heterologous poly(A)+ RNA in Acetabularia cytoplasm.




© The Company of Biologists Ltd 1982