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Journal of Cell Science, Vol 5, 583-602, Copyright © 1969 by Company of Biologists

Submitted on February 13, 1969

Cytology and Growth Characteristics of Human Tumour Astrocytes Transformed by Rous Sarcoma Virus

E. H. MACINTYRE 1, R. A. GRIMES 1, and A. E. VATTER 1

1 Department of Pathology, University of Colorado Medical Center, and Webb-Waring Institute for Medical Research Denver, Colorado 80220, U.S.A.

Human tumour astrocytes (118MG) were exposed in vitro to the Engelbreth-Holm strain of Rous sarcoma virus at a multiplicity of infection of one. Morphological transformation of the cells in 60-mm plates was complete in 9-11 weeks after viral exposure. The transformed cells (EH-118 MG) grew as a monolayer, upon which nests of rounded cells developed. From these nests, rounded cells shed into the medium and in turn on seeding formed a monolayer and repeated the cycle. The transformed cells contained the viral group-specific antigen in the cytoplasm, and produced Rous sarcomas in chickens. No complete virus was demonstrated in the mammalian cells. Electron micrographs of the parent 118 MG and transformed EH-118 MG cells gave further details of the structure of cytoplasmic protrusions which were formed in some of the free EH-118 MG but not in the 118 MG cells. Their nature is completely unknown.

Submitted on February 13, 1969




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© The Company of Biologists Ltd 1969