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Journal of Cell Science, Vol 48, Issue 1 281-290, Copyright © 1981 by Company of Biologists

JOURNAL ARTICLES

Effects of steroids and different culture media on cell cycle of the androgen-sensitive human cell line NHIK3025

K Ostgaard, E Wibe, T Lindmo and KB Eik-Nes

The median cell cycle of synchronized NHIK3025 cells grown in Eagle's MEM with 10% foetal calf serum was 23.6 h, compared to 18 h observed earlier in Puck's E2a medium with 30% serum (20% human and 10% horse). This difference is due to prolongation of both G1 and G2 in the MEM type medium. Testosterone and 4-androstene-3 beta, 17 beta-diol reduced the cell cycle of synchronized populations of NHIK3025 cultured in MEM type medium. Dexamethasone gave cell cycle prolongation while estradiol had no effect. These results are in accordance with steroid-induced changes in growth of asynchronous cell populations observed earlier. The androgen growth stimulation was partly due to a shortening of G2 but was not exclusively located in one particular phase of the cell cycle.




© The Company of Biologists Ltd 1981