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Journal of Cell Science, Vol 47, Issue 1 227-236, Copyright © 1981 by Company of Biologists

JOURNAL ARTICLES

The formation and orientation of brush border vesicles from rat duodenal mucosa

PR Hearn, RG Russell and J Farmer

An electron-microscopic study of various fractions taken during a newly developed method for preparation of brush border vesicles from rat duodenum, has shown that vesiculation of microvilli can occur in situ on the brush border membrane. This mode of formation ensures that the vesicle membranes are oriented as in vivo and that they are "right-side-out". The disruption of core protein-fibres appears to be a prerequisite for vesicle formation.


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© The Company of Biologists Ltd 1981