spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Green, J. R.
Right arrow Articles by Northcote, D. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Green, J. R.
Right arrow Articles by Northcote, D. H.

Journal of Cell Science, Vol 40, Issue 1 235-244, Copyright © 1979 by Company of Biologists

JOURNAL ARTICLES

Location of fucosyl transferases in the membrane system of maize root cells

JR Green and DH Northcote

There are two fucosyl transferase activities present within the endomembranes of the cells of maize root-tips. One transfers fucose to polyprenyl phosphate and occurs in the endoplasmic reticulum, the second transfers fucose probably to polysaccharide or glycoprotein. In order to show an association of this second fucosyl transferase activity with the endoplasmic reticulum as well as the Golgi apparatus, a method of fractionating the membranes in a discontinuous sucrose gradient was used. Membranes were prepared in the presence of Mg2+, which maintained the attachment of ribosomes to the endoplasmic reticulum, and also in the presence of EDTA, which removed most of the ribosome complex. This caused a shift in density of these membranes. Two types of experiments were carried out; either maize roots were incubated in L-[1-3H]fucose and then membranes prepared and the amount of polymer synthesized in vivo determined or isolated membranes were incubated with GDP-L-[U-14C]fucose in vitro and the amount of polymer synthesized was found. The results showed that the Golgi apparatus had the highest amount of this fucosyl transferase activity, but there was a significant amount of activity associated with the endoplasmic reticulum and the latter was shifted in the sucrose gradient depending on the conditions used.


This article has been cited by other articles:


Home page
 ANN BOT (LOND)Home page
S. SINHA ROY, B. MITTRA, S. SHARMA, T. K. DAS, and C. R. BABU
Detection of Root Mucilage Using an Anti-fucose Antibody
Ann. Bot., March 1, 2002; 89(3): 293 - 299.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 1979