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Journal of Cell Science, Vol 4, 369-379, Copyright © 1969 by Company of Biologists

Submitted on May 20, 1968
Revised on September 16, 1968

Particles Present in the Haemolymph And Defensive Secretions of Insects

D. KAY 1, MIRIAM ROTHSCHILD 2, and R. APLIN 3

1 Sir William Dunn School of Pathology, Oxford
2 Ashton, Peterborough
3 Dyson Perrins Laboratory, Oxford, England

Defensive fluids obtained from the Garden Tiger, Scarlet Tiger and Burnet Moths and from the Monarch Butterfly and the Seven-Spot Ladybird Beetle, haemolymphs taken from the wasp, the Burying Beetle and the Rat Flea, and fluids from the eggs of the Garden Tiger, Gypsy Moth and Oak Eggar Moth were examined in the electron microscope after negative staining.

Except for the ladybird all the defensive fluids, haemolymphs and egg fluids contained particles 100-150 Å across which frequently had rectangular or square outlines. In some species the particles were occasionally arranged in groups of 2 and 4 and in the egg fluid of the Garden Tiger they were arranged in rows which were aligned side by side to form strands of considerable length.

The particles are believed to be protein. They correspond in size to the 16 s component of the haemocyanins of Limulus and Homarus and the protein particles found in Calliphora by other workers.

Submitted on May 20, 1968
Revised on September 16, 1968







© The Company of Biologists Ltd 1969