spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by McCready, S. J.
Right arrow Articles by Cook, P. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by McCready, S. J.
Right arrow Articles by Cook, P. R.

Journal of Cell Science, Vol 39, Issue 1 53-62, Copyright © 1979 by Company of Biologists

JOURNAL ARTICLES

Electron-microscopy of intact nuclear DNA from human cells

SJ McCready, A Akrigg and PR Cook

Structures retaining many of the morphological features of nuclei may be released by lysing human cells in a non-ionic detergent and 2 M NaCl. Such nucleoids contain all the nuclear DNA packaged within a flexible cage of RNA and protein. HeLa nucleoids have been spread at an air-water interface and viewed in the electron microscope. A tangled network of superhelical fibres surrounds the collapsed cage. Irradiation with gamma-rays abolishes supercoiling and treatment with the untwisting enzyme or a low concentration of ethidium reduces it. A high concentration of ethidium induces supertwisting. The nuclear DNA of higher cells can be isolated naked, supercoiled and intact.


This article has been cited by other articles:


Home page
 JCBHome page
K. Byrd and V. G. Corces
Visualization of chromatin domains created by the gypsy insulator of Drosophila
J. Cell Biol., August 18, 2003; 162(4): 565 - 574.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
H. Ma, A. J. Siegel, and R. Berezney
Association of Chromosome Territories with the Nuclear Matrix: Disruption of Human Chromosome Territories Correlates with the Release of a Subset of Nuclear Matrix Proteins
J. Cell Biol., August 9, 1999; 146(3): 531 - 542.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 1979