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Journal of Cell Science, Vol 27, Issue 1 289-301, Copyright © 1977 by Company of Biologists

JOURNAL ARTICLES

Distribution of anionic sites on surface of B cell granule and plasma membranes: a study using cationic ferritin

SL Howell and M Tyhurst

The distribution of anionic sites on the membranes of rat pancreatic B cells and of their storage granules has been studied by the use of a visual probe of cationic ferritin. Membranes of isolated storage granules possessed a net negative charge which was apparently evenly distributed; the number of anionic sites was not markedly altered by prior incubation of the granules with neuraminidase or with 10(-5) to 2 X 10(-3) M calcium chloride. Distribution of charges along B cell plasma membranes was less uniform but was similarly unaffected by alterations of calcium concentration, or by neuraminidase treatment. However, during the fusion of plasma membrane and granule membrane which occurs in exocytosis, the emerging granule membrane was found to be devoid of anionic sites. The implications of these findings for the regulation of insulin secretion by exocytosis are discussed.




© The Company of Biologists Ltd 1977