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Journal of Cell Science, Vol 25, Issue 1 17-37, Copyright © 1977 by Company of Biologists

JOURNAL ARTICLES

Gene transfer by means of cell fusion I. Statistical mapping of the human X-chromosome by analysis of radiation-induced gene segregation

SJ Goss and H Harris

Hybrid cells were obtained by virus-induced fusion of hamster cells with irradiated human cells. The analysis of such hybrids permits a study of the effects of lethal doses of radiation on human cells and provides a method of sub-chromosomal genetic mapping that is independent of karyological analysis. Radiation-induced chromosome exchanges are shown to be extremely localized, and a map of 4 X-linked genes is presented.


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© The Company of Biologists Ltd 1977