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Journal of Cell Science, Vol 22, Issue 2 413-425, Copyright © 1976 by Company of Biologists

JOURNAL ARTICLES

Purification of gibberellic acid-induced lysosomes from wheat aleurone cells

RA Gibson and LG Paleg

Using isopycnic density gradient centrifugation, lysosomes were concentrated in a single region of a sucrose-Ficoll gradient (p = 1-10 g cm-3), well separated from most other cell organelles. Gibberellic acid-induced lysosomes were found to be rich in alpha-amylase and protease but not ribonuclease. The lysosomal band also contained a majority of the NADH2-cytochrome c reductase, a marker enzyme for endoplasmic reticulum, found in the gradient. Examination of electron micrographs revealed that a purified band of lyosomes contained at least 3 vesicle types, ranging in size from 0-1 to 0-5 mum. The significance of these findings to proposed mechanisms of action of gibberellic acid is discussed.




© The Company of Biologists Ltd 1976