spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cook, P. R.
Right arrow Articles by Brazell, I. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cook, P. R.
Right arrow Articles by Brazell, I. A.

Journal of Cell Science, Vol 22, Issue 2 287-302, Copyright © 1976 by Company of Biologists

JOURNAL ARTICLES

Conformational constraints in nuclear DNA

PR Cook and IA Brazell

We have investigated DNA superstructure in a wide range of nuclei of higher cells by gently lysing cells to release structures that resemble nuclei but are depleted of nuclear proteins. The sedimentation properties of these structures, which we call nucleoids, have been examined in sucrose gradients containing the intercalating agent, ethidium. The sedimentation rate of nucleoids derived from the growing cells of mammals, birds, amphibians and insects varies in the manner characteristic of circular and superhelical molecules of DNA. These characteristic changes in sedimentation rate are abolished by irradiating the nucleoids with low doses of gamma-rays, a procedure known to introduce single-strand scissions into DNA. We have also investigated by similar means DNA superstructure in nucleoids derived from a variety of different chick cells. Nucleoids derived from adult hen erythrocytes differ from the other nucleoids studied in that their sedimentation rate does not vary in the manner characteristic of supercoiled DNA.


This article has been cited by other articles:


Home page
 MutagenesisHome page
A. R. Collins, A. A. Oscoz, G. Brunborg, I. Gaivao, L. Giovannelli, M. Kruszewski, C. C. Smith, and R. Stetina
The comet assay: topical issues
Mutagenesis, May 1, 2008; 23(3): 143 - 151.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
D. Marenduzzo, K. Finan, and P. R. Cook
The depletion attraction: an underappreciated force driving cellular organization
J. Cell Biol., December 4, 2006; 175(5): 681 - 686.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
I. Faro-Trindade and P. R. Cook
A Conserved Organization of Transcription during Embryonic Stem Cell Differentiation and in Cells with High C Value
Mol. Biol. Cell, July 1, 2006; 17(7): 2910 - 2920.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
V. T. Solovyan, Z. A. Bezvenyuk, A. Salminen, C. A. Austin, and M. J. Courtney
The Role of Topoisomerase II in the Excision of DNA Loop Domains during Apoptosis
J. Biol. Chem., June 7, 2002; 277(24): 21458 - 21467.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
A.-L. Paul and R. J. Ferl
Higher Order Chromatin Structures in Maize and Arabidopsis
PLANT CELL, August 1, 1998; 10(8): 1349 - 1360.
[Abstract] [Full Text]

Home page
 J. Cell Biol.Home page
I. de Belle, S. Cai, and T. Kohwi-Shigematsu
The Genomic Sequences Bound to Special AT-rich Sequence-binding Protein 1 (SATB1) In Vivo in Jurkat T Cells Are Tightly Associated with the Nuclear Matrix at the Bases of the Chromatin Loops
J. Cell Biol., April 20, 1998; 141(2): 335 - 348.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
P. Cook
A chromomeric model for nuclear and chromosome structure
J. Cell Sci., January 9, 1995; 108(9): 2927 - 2935.
[Abstract] [PDF]


© The Company of Biologists Ltd 1976