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Journal of Cell Science, Vol 19, Issue 1 157-167, Copyright © 1975 by Company of Biologists

JOURNAL ARTICLES

Localization of lectins in legume cotyledons

AE Clarke, RB Knox and MA Jermyn

High-resolution techniques for the localization of lectins are described. Concanavalin A (Con A) and phytohaemagglutinin (PHA) are localized using a fluorescent method with (FITC)-labelled immunoglobulins which bind to the lectins in sections of jack and red kidney bean cotyledons. Specificity is defined by the use of specific sugar inhibitors. Both Con A and PHA are found in cytoplasmic sites. Lectins with beta-glycoside specificity are detected with red-coloured artificial carbohydrate antigens. The beta-galactosyl and beta-glucosyl antigens bind specifically to clusters of spherical bodies in the intercellular spaces, to cell wall sites, and to the periphery of the cytoplasm associated with the cell membrane.


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D. W. FOUNTAIN, D. E. FOARD, W. D. REPLOGLE, and W. K. YANG
Lectin Release by Soybean Seeds
Science, September 16, 1977; 197(4309): 1185 - 1187.
[Abstract] [PDF]


© The Company of Biologists Ltd 1975