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Journal of Cell Science, Vol 14, 85-103, Copyright © 1974 by Company of Biologists

Submitted on May 31, 1973

Incorporation of [3H]Uridine and [3H]Thymidine During the Phase of Nucleolar Multiplication in Xenopus Laevis Oögenesis: A High-Resolution Autoradiographic Study

P. VAN GANSEN 1 and A. SCHRAM 1

1 Laboratoire de Cytologie et Embryologie moléculaires, Université libre de Bruxelles Belgium

The nucleoli of the premeiotic, pachytene and young diplotene (A, B, C, up to 100µm diameter) oocytes are heavily labelled after 7 h of [3H]uridine incorporation, which is in good agreement with the hypothesis that nucleolar multiplication occurs throughout all these early stages of oogenesis. The total labelling of the nucleolar, nuclear and cytoplasmic volumes has been calculated from the uridine high-resolution autoradiograms and the estimated amounts are discussed. The chromatin of several premeiotic oocytes and the cap of all the pachytene oocytes are strongly labelled after 7 h of [3H]thymidine incorporation. We did not find any thymidine incorporation in the young A, B or C diplotene oocytes.

Submitted on May 31, 1973






© The Company of Biologists Ltd 1974