spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by HANKE, D. E.
Right arrow Articles by NORTHCOTE, D. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by HANKE, D. E.
Right arrow Articles by NORTHCOTE, D. H.

Journal of Cell Science, Vol 14, 29-50, Copyright © 1974 by Company of Biologists

Submitted on May 9, 1973

Cell Wall Formation by Soybean Callus Protoplasts

D. E. HANKE 1 and D. H. NORTHCOTE 2

1 Department of Biochemistry, University of Cambridge, Cambridge CB2 1QW, England; School of Botany, University of Cambridge, Cambridge, England.
2 Department of Biochemistry, University of Cambridge, Cambridge CB2 1QW, England

Protoplasts isolated from soybean (Glycine max (L.) Merr.) callus and cultured for 4o h developed a wall which could be seen on plasmolysis. They changed shape, producing buds, and became osmotically stable. Analysis after feeding radioactive glucose showed that the regenerated wall was devoid of pectin which was in the medium. Budding occurs because of the loss of pectin and the observations assist understanding of wall expansion and growth in normal plant cells. The complete molecule of weakly acidic pectinic acid was secreted, precluding the possibility of its assembly in the wall.

Submitted on May 9, 1973






© The Company of Biologists Ltd 1974