spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online 3 April 2007
doi: 10.1242/jcs.03439

Journal of Cell Science 120, 1596-1606 (2007)
Published by The Company of Biologists 2007
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jcs.03439v1
120/9/1596    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Related articles in JCS
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chandramouly, G.
Right arrow Articles by Lelièvre, S. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chandramouly, G.
Right arrow Articles by Lelièvre, S. A.

Research Article

The control of tissue architecture over nuclear organization is crucial for epithelial cell fate

Gurushankar Chandramouly1, Patricia C. Abad1, David W. Knowles2 and Sophie A. Lelièvre1,*

1 Department of Basic Medical Sciences and Cancer Center, Purdue University, 625 Harrison Street, West Lafayette, IN 47907-2026, USA
2 Life Sciences Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, CA 94720, USA

* Author for correspondence (e-mail: lelievre{at}purdue.edu)

Accepted 23 February 2007

The remodeling of nuclear organization during differentiation and the dramatic alteration of nuclear organization associated with cancer development are well documented. However, the importance of tissue architecture in the control of nuclear organization remains to be determined. Differentiation of mammary epithelial cells into functional tissue structures, in three-dimensional culture, is characterized by a specific tissue architecture (i.e. a basoapical polarity axis), cell cycle exit and maintenance of cell survival. Here we show that induction of partial differentiation (i.e. basal polarity only, cell cycle exit and cell survival) by epigenetic mechanisms in malignant breast cells is sufficient to restore features of differentiation-specific nuclear organization, including perinucleolar heterochromatin, large splicing factor speckles, and distinct nuclear mitotic apparatus protein (NuMA) foci. Upon alteration of nuclear organization using an antibody against NuMA, differentiated non-neoplastic cells undergo apoptosis, whereas partially differentiated malignant cells enter the cell cycle. Non-neoplastic cells cultured under conditions that prevent the establishment of apical polarity also enter the cell cycle upon NuMA antibody treatment. These findings demonstrate that the differentiation status rather than the non-neoplastic or neoplastic origin of cells controls nuclear organization and suggest a link between nuclear organization and epigenetic mechanisms dictated by tissue architecture for the control of cell behavior.

Key words: Mammary epithelial differentiation, Nuclear structure, Proliferation, Tumor reversion, NuMA, Three dimensional cell culture


Related articles in JCS:

Epithelial differentiation goes nuclear

JCS 2007 120: 904. [Full Text]  



This article has been cited by other articles:


Home page
 J. Cell Biol.Home page
K. J. Meaburn and T. Misteli
Locus-specific and activity-independent gene repositioning during early tumorigenesis
J. Cell Biol., January 10, 2008; 180(1): 39 - 50.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2007