spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online 20 March 2007
doi: 10.1242/jcs.003954

Journal of Cell Science 120, 1383-1392 (2007)
Published by The Company of Biologists 2007
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary Material
Right arrow All Versions of this Article:
jcs.003954v1
120/8/1383    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bax, D. V.
Right arrow Articles by Kielty, C. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bax, D. V.
Right arrow Articles by Kielty, C. M.

Research Article

Cell adhesion to fibrillin-1: identification of an Arg-Gly-Asp-dependent synergy region and a heparin-binding site that regulates focal adhesion formation

Daniel V. Bax1,2, Yashithra Mahalingam3, Stuart Cain2, Kieran Mellody2, Lyle Freeman2, Kerri Younger2, C. Adrian Shuttleworth2, Martin J. Humphries2, John R. Couchman3 and Cay M. Kielty1,2,*

1 UK Centre for Tissue Engineering, Faculty of Life Sciences, University of Manchester, Manchester, M13 9PT, UK
2 Wellcome Trust Centre for Cell-Matrix Research, Faculty of Life Sciences, University of Manchester, Manchester, M13 9PT, UK
3 Faculty of Medicine, Imperial College London, Exhibition Road, London, SW7 2AZ, UK

* Author for correspondence (e-mail: cay.kielty{at}manchester.ac.uk)

Accepted 5 February 2007

We have defined the molecular basis of cell adhesion to fibrillin-1, the major structural component of extracellular microfibrils that are associated with elastic fibres. Using human dermal fibroblasts, and recombinant domain swap fragments containing the Arg-Gly-Asp motif, we have demonstrated a requirement for upstream domains for integrin-{alpha}5beta1-mediated cell adhesion and migration. An adjacent heparin-binding site, which supports focal adhesion formation, was mapped to the fibrillin-1 TB5 motif. Site-directed mutagenesis revealed two arginine residues that are crucial for heparin binding, and confirmed their role in focal adhesion formation. These integrin and syndecan adhesion motifs juxtaposed on fibrillin-1 are evolutionarily conserved and reminiscent of similar functional elements on fibronectin, highlighting their crucial functional importance.

Key words: Fibrillin-1, Cell adhesion, Integrins, Heparin, Syndecan-4, Fibronectin




This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
R. Kinsey, M. R. Williamson, S. Chaudhry, K. T. Mellody, A. McGovern, S. Takahashi, C. A. Shuttleworth, and C. M. Kielty
Fibrillin-1 microfibril deposition is dependent on fibronectin assembly
J. Cell Sci., August 15, 2008; 121(16): 2696 - 2704.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
S. E. McGowan, A. J. Holmes, R. P. Mecham, and T. M. Ritty
Arg-Gly-Asp-Containing Domains of Fibrillins-1 and -2 Distinctly Regulate Lung Fibroblast Migration
Am. J. Respir. Cell Mol. Biol., April 1, 2008; 38(4): 435 - 445.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
D. DiCara, A. Burman, S. Clark, S. Berryman, M. J. Howard, I. R. Hart, J. F. Marshall, and T. Jackson
Foot-and-Mouth Disease Virus Forms a Highly Stable, EDTA-Resistant Complex with Its Principal Receptor, Integrin {alpha}v 6: Implications for Infectiousness
J. Virol., February 1, 2008; 82(3): 1537 - 1546.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2007