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First published online September 18, 2007
doi: 10.1242/10.1242/jcs.015883

Journal of Cell Science 120, 3179-3187 (2007)
Published by The Company of Biologists 2007
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Research Article

The AAA-ATPase FIGL-1 controls mitotic progression, and its levels are regulated by the CUL-3MEL-26 E3 ligase in the C. elegans germ line

Sarah Luke-Glaser1, Lionel Pintard2,*,{ddagger}, Mike Tyers2 and Matthias Peter1,{ddagger}

1 Institute of Biochemistry, HPM G8, ETH Hönggerberg, 8093 Zürich, Switzerland
2 Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto M5G1X5, Canada

{ddagger} Authors for correspondence (e-mails: pintard.lionel{at}ijm.jussieu.fr; matthias.peter{at}bc.biol.ethz.ch)

Accepted 10 July 2007

Members of the AAA-ATPase (ATPases associated with diverse cellular activities) family use the energy from ATP hydrolysis to disrupt protein complexes involved in many cellular processes. Here, we report that FIGL-1 (Fidgetin-like 1), the single Caenorhabditis elegans homolog of mammalian fidgetin and fidgetin-like 1 AAA-ATPases, controls progression through mitosis in the germ line and the early embryo. Loss of figl-1 function leads to the accumulation of mitotic nuclei in the proliferative zone of the germ line, resulting in sterility owing to depletion of germ cells. Like the AAA-ATPase MEI-1 (also known as katanin), FIGL-1 interacts with microtubules and with MEL-26, a specificity factor of CUL-3-based E3 ligases involved in targeting proteins for ubiquitin-dependent degradation by the 26S proteasome. In the germ line, FIGL-1 is enriched in nuclei of mitotic cells, but it disappears at the transition into meiosis. Conversely, MEL-26 expression is low in nuclei of the mitotic zone and induced during meiosis. FIGL-1 accumulates in the germ line and spreads to the meiotic zone after inactivation of mel-26 or cul-3 in vivo. We conclude that degradation of FIGL-1 by the CUL-3MEL-26 E3 ligase spatially restricts FIGL-1 function to mitotic cells, where it is required for correct progression through mitosis.

Key words: Ubiquitin-dependent degradation, Germ line, Embryo, Cullin, Fidgetin, Fidgetin-like


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JCS 2007 120: 1802. [Full Text]  





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