spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online 13 June 2007
doi: 10.1242/jcs.002345

Journal of Cell Science 120, 2284-2294 (2007)
Published by The Company of Biologists 2007
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jcs.002345v1
120/13/2284    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ding, B.
Right arrow Articles by Cho, N. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ding, B.
Right arrow Articles by Cho, N. H.

Research Article

Role of p53 in antioxidant defense of HPV-positive cervical carcinoma cells following H2O2 exposure

Boxiao Ding1,2, Sung Gil Chi3, Se Heon Kim4, Suki Kang1, Jae Ho Cho5, Dong Su Kim6 and Nam Hoon Cho1,2,*

1 Department of Pathology, Yonsei University College of Medicine, Seoul, Korea
2 Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul, Korea
3 School of Life Sciences and Biotechnology, Korea University, Seoul, Korea
4 Department of Otorhinolaryngology, Yonsei University College of Medicine, Seoul, Korea
5 Department of Radiation Oncology, Yonsei University College of Medicine, Seoul, Korea
6 Genomine Research Division, Genomine Inc., Pohang, Korea

* Author for correspondence (e-mail: cho1988{at}yumc.yonsei.ac.kr)

Accepted 26 April 2007

In HPV-positive cervical carcinoma cells, p53 protein is functionally antagonized by the E6 oncoprotein. We investigated a possible role of p53 in antioxidant defense of HPV-positive cervical cancer cell lines. We found that SiHa cells containing integrated HPV 16 had higher expression of p53 and exhibited the greatest resistant to H2O2-induced oxidative damage, compared with HeLa, CaSki and ME180 cell lines. Downregulation of p53 resulted in the inhibition of p53-regulated antioxidant enzymes and elevated intracellular ROS in SiHa cells. By contrast, the ROS level was not affected in HeLa, CaSki and ME180 cell lines after inhibition of the p53 protein. Under mild or severe H2O2-induced stress, p53-deficient SiHa cells exhibited much higher ROS levels than control SiHa cells. Furthermore, we analyzed cell viability and apoptosis after H2O2 treatment and found that p53 deficiency sensitized SiHa cells to H2O2 damage. Inhibition of p53 resulted in excessive oxidation of DNA; control SiHa cells exhibited a more rapid removal of 8-oxo-7,8-dihydro-2'-deoxyguanosine from DNA compared with p53-deficient SiHa cells exposed to the same level of H2O2 challenge. These data collectively show that endogenous p53 in SiHa cells has an antioxidant function and involves in the reinforcement of the antioxidant defense.

Key words: p53, SiHa, H2O2, ROS, Oxidative stress, RNA interference (RNAi)




This article has been cited by other articles:


Home page
 IOVSHome page
J. C. O'Connor, D. M. Wallace, C. J. O'Brien, and T. G. Cotter
A Novel Antioxidant Function for the Tumor-Suppressor Gene p53 in the Retinal Ganglion Cell
Invest. Ophthalmol. Vis. Sci., October 1, 2008; 49(10): 4237 - 4244.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2007