spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online 13 June 2007
doi: 10.1242/jcs.007203

Journal of Cell Science 120, 2232-2240 (2007)
Published by The Company of Biologists 2007
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow An erratum has been published
Right arrow All Versions of this Article:
jcs.007203v1
120/13/2232    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Related articles in JCS
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bracci, L.
Right arrow Articles by Treves, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bracci, L.
Right arrow Articles by Treves, S.

Research Article

Ca2+ signaling through ryanodine receptor 1 enhances maturation and activation of human dendritic cells

Laura Bracci1,*,{ddagger}, Mirko Vukcevic3,*, Giulio Spagnoli1, Sylvie Ducreux2, Francesco Zorzato3 and Susan Treves2,§

1 Institute of Surgical Research, Basel University Hospital, Hebelstrasse 20, 4031 Basel, Switzerland
2 Departments of Anesthesia and Research, Basel University Hospital, Hebelstrasse 20, 4031 Basel, Switzerland
3 Department of Experimental and Diagnostic Medicine, General Pathology Section, University of Ferrara, 44100 Ferrara, Italy

§ Author for correspondence (e-mail: susan.treves{at}unibas.ch)

Accepted 5 May 2007

Increases in intracellular Ca2+ concentration accompany many physiological events, including maturation of dendritic cells, professional antigen-presenting cells characterized by their ability to migrate to secondary lymphoid organs where they initiate primary immune responses. The mechanism and molecules involved in the early steps of Ca2+ release in dendritic cells have not yet been defined. Here we show that the concomitant activation of ryanodine receptor-induced Ca2+ release together with the activation of Toll-like receptors by suboptimal concentrations of microbial stimuli provide synergistic signals, resulting in dendritic cell maturation and stimulation of T cell functions. Furthermore, our results show that the initial intracellular signaling cascade activated by ryanodine receptors is different from that induced by activation of Toll-like receptors. We propose that under physiological conditions, especially when low suboptimal amounts of Toll-like receptor ligands are present, ryanodine receptor-mediated events cooperate in bringing about dendritic cell maturation.

Key words: Dendritic cell, Maturation, Ryanodine receptor, Signaling


Related articles in JCS:

Coffee break for dendritic cell Ca2+

JCS 2007 120: 1303. [Full Text]  





© The Company of Biologists Ltd 2007