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Journal of Cell Science, Vol 12, 617-629, Copyright © 1973 by Company of Biologists

Submitted on June 27, 1972

The Effects of Adapting Human Diploid Cells to Grow in Glutamic Acid Media on Cell Morphology, Growth and Metabolism

J. B. GRIFFITHS 1

1 Microbiological Research Establishment, Porton, Salisbury, Wiltshire, England

Two lines of human diploid cells, the W1-38 and MRC-5, were adapted to utilize glutamic acid in place of glutamine. This adaptation resulted in (a) more cells per unit culture area, (b) an alteration in cell size and protein content, (c) a morphological change of the cells from fibroblastic to epithelial-like, and (d) increased metabolic activity. Changes in the agglutinability of adapted cells by 3 lectins together with the results from polyacrylamide gel electrophoresis could be interpreted as a change in plasma membrane structure. Comparative studies of unadapted, adapted and transformed cells showed that adaptation to glutamic acid produced cells with a metabolism and amino acid uptake similar to those of transformed cells. These changes were reversible and were not accompanied by any apparent karyological change. The significance of these results for the study of density-dependent inhibition of growth is discussed.

Submitted on June 27, 1972




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R. McDermott and M Butler
Uptake of glutamate, not glutamine synthetase, regulates adaptation of mammalian cells to glutamine-free medium
J. Cell Sci., January 1, 1993; 104(1): 51 - 58.
[Abstract] [PDF]


© The Company of Biologists Ltd 1973