spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by WIENER, F.
Right arrow Articles by HARRIS, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by WIENER, F.
Right arrow Articles by HARRIS, H.

Journal of Cell Science, Vol 12, 253-261, Copyright © 1973 by Company of Biologists

Submitted on July 7, 1972

The Analysis of Malignancy by Cell Fusion

IV. Hybrids Between Tumour Cells and a Malignant L Cell Derivative

F. WIENER 1, G. KLEIN 1, and H. HARRIS 2

1 Department of Tumour Biology, Karolinska Institutet 104 01 Stockholm 60, Sweden
2 Sir William Dunn School of Pathology, University of Oxford Oxford OX1 3RE, England

Previous experiments showed that when a range of different highly malignant mouse tumour cells were fused with L cell derivatives of low tumorigenicity, the resulting hybrid cells, so long as they retained something close to the complete chromosome sets of both parent cells, had little or no ability to grow progressively in vivo. Tumours arising from the injection of such hybrids were produced not by progressive growth of the cells injected, but by selective overgrowth of cells from which certain specific, but as yet unidentified, chromosomes had been eliminated. In the present experiments the same malignant mouse tumour cells were fused with a highly malignant L cell derivative selected from the wild type cell population by passage through the animal. In all cases the resulting hybrid cells were found to be highly malignant; and the chromosome constitutions of the tumours arising from the injection of these hybrids were not significantly different from those of the cells injected. These findings confirm the interpretation given to the previous work; the L cell derivatives of low tumorigenicity contribute to the hybrid cells some factor, linked to specific chromosomes, that suppresses the malignant character of the tumour cell, and this suppression is removed, with consequent reappearance of the malignant phenotype, when certain chromosomes are eliminated.

Submitted on July 7, 1972




This article has been cited by other articles:


Home page
 ScienceHome page
P. Nowell
The clonal evolution of tumor cell populations
Science, October 1, 1976; 194(4260): 23 - 28.
[Abstract] [PDF]

Home page
 Mol. Biol. CellHome page
K. Hu, T. Mann, B. Striepen, C. J. M. Beckers, D. S. Roos, and J. M. Murray
Daughter Cell Assembly in the Protozoan Parasite Toxoplasma gondii
Mol. Biol. Cell, February 1, 2002; 13(2): 593 - 606.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 1973