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First published online 21 February 2006
doi: 10.1242/jcs.02816

This Article Figures Only Full Text Full Text (PDF) Supplementary Material All Versions of this Article: jcs.02816v1 119/6/1026    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in JCS Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Dellaire, G. Articles by Bazett-Jones, D. P. Search for Related Content PubMed PubMed Citation Articles by Dellaire, G. Articles by Bazett-Jones, D. P.

The number of PML nuclear bodies increases in early S phase by a fission mechanism

Graham Dellaire^*, Reagan W. Ching^*, Hesam Dehghani, Ying Ren and David P. Bazett-Jones

Programme in Cell Biology, The Hospital for Sick Children, 555 University Avenue, Toronto, Ontario, M5G 1X8 Canada

Author for correspondence (e-mail: dbjones{at}sickkids.ca)

Accepted 30 November 2005

Promyelocytic leukemia (PML) nuclear bodies have been implicated in a variety of cellular processes including apoptosis, tumour suppression, anti-viral response, DNA repair and transcriptional regulation. PML nuclear bodies are both positionally and structurally stable over extended periods of interphase. As demonstrated in this study, the structural stability is lost as cells enter S phase, evidenced both by distortions in shape and by fission and fusion events. At the end of this period of structural instability, the number of PML nuclear bodies has increased by a factor of twofold. Association of the fission products with chromatin implies that the PML nuclear bodies respond to changes in chromatin organisation or topology, and thus could play a role in monitoring genome integrity during DNA synthesis or in the continued maintenance of functional chromosomal domains prior to mitosis.

Key words: PML nuclear body, DNA synthesis, Electron spectroscopic imaging, Particle tracking, Nuclear organisation

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