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First published online February 22, 2006
doi: 10.1242/10.1242/jcs.02796

Journal of Cell Science 119, 933-942 (2006)
Published by The Company of Biologists 2006
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Research Article

Upregulation of chondroitin 6-sulphotransferase-1 facilitates Schwann cell migration during axonal growth

Jun Liu1, Chi-Ho Chau1,2, Hengying Liu1, Benjamin R. Jang1, Xiaoguang Li1,3, Ying-Shang Chan2 and Daisy K. Y. Shum1,*

1 Department of Biochemistry, Faculty of Medicine, The University of Hong Kong, 21 Sassoon Road, Pokfulam, Hong Kong, China
2 Department of Physiology, Faculty of Medicine, The University of Hong Kong, 21 Sassoon Road, Pokfulam, Hong Kong, China
3 Department of Neurology, Peking Union Medical College Hospital, Beijing, China

* Author for correspondence (e-mail: shumdkhk{at}hkucc.hku.hk)

Accepted 15 November 2005

Cell migration is central to development and post-traumatic regeneration. The differential increase in 6-sulphated chondroitins during axonal growth in both crushed sciatic nerves and brain development suggests that chondroitin 6-sulphotransferase-1 (C6ST-1) is a key enzyme that mediates cell migration in the process. We have cloned the cDNA of the C6ST-1 gene (C6st1) (GenBank accession number AF178689) from crushed sciatic nerves of adult rats and produced ribonucleotide probes accordingly to track signs of 6-sulphated chondroitins at the site of injury. We found C6st1 mRNA expression in Schwann cells emigrating from explants of both sciatic nerve segments and embryonic dorsal root ganglia. Immunocytochemistry indicated pericellular 6-sulphated chondroitin products around C6ST-1-expressing frontier cells. Motility analysis of frontier cells in cultures subjected to staged treatment with chondroitinase ABC indicated that freshly produced 6-sulphated chondroitin moieties facilitated Schwann cell motility, unlike restrictions resulting from proteoglycan interaction with matrix components. Sciatic nerve crush provided further evidence of in vivo upregulation of the C6ST-1 gene in mobile Schwann cells that guided axonal regrowth 1-14 days post crush; downregulation then accompanied declining mobility of Schwann cells as they engaged in the myelination of re-growing axons. These findings are the first to identify upregulated C6st1 gene expression correlating with the motility of Schwann cells that guide growing axons through both developmental and injured environments.

Key words: Chondroitin sulphate proteoglycans, Development, Dorsal root ganglion, Sciatic nerve crush, Nerve regeneration




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[Abstract] [Full Text] [PDF]


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