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First published online 21 November 2006
doi: 10.1242/jcs.03298

Journal of Cell Science 119, 5067-5076 (2006)
Published by The Company of Biologists 2006
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Research Article

An unexpected role for keratin 10 end domains in susceptibility to skin cancer

Jiangli Chen1, Xing Cheng1, Maria Merched-Sauvage1, Carlos Caulin1, Dennis R. Roop1,2 and Peter J. Koch1,2,*

1 Department of Dermatology, Baylor College of Medicine, Houston, TX 77030, USA
2 Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX 77030, USA

* Author for correspondence (e-mail: pkoch{at}bcm.tmc.edu)

Accepted 9 October 2006

Keratin 10 (K10) is a type I keratin that is expressed in post-mitotic suprabasal keratinocytes of the skin. Based on cell culture experiments and transgenic mouse studies, it has been proposed that K10 suppresses cell proliferation and tumor formation in the skin. Furthermore, the ability of K10 to suppress cell proliferation was mapped to its unique N- and C-terminal protein domains. In the present study, we modified the endogenous keratin 14 (K14) gene of mice using a knock-in approach to encode a chimeric keratin that consists of the K14 rod domain fused to the K10 head and tail domains (K1014chim). This transgene was expressed in the basal layer of the epidermis and the outer root sheath of hair follicles. Unexpectedly, we found that the K10 end domains had no effect on basal keratinocyte proliferation in vivo. Moreover, when subjected to a chemical skin carcinogenesis protocol, papilloma formation in mutant mice was accelerated instead of being inhibited. Our data suggest that the increased tumor susceptibility of K1014chim mice is in part due to a suppression of apoptosis in mutant keratinocytes. Our results support the notion that intermediate filaments, in addition to their function as cytoskeletal components, affect tumor susceptibility of epithelial cells.

Key words: Intermediate filaments, Keratin 10, Keratin 14, Apoptosis, Skin carcinogenesis, Keratinocytes


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Keratin 10 - cancer in the end

JCS 2006 119: 2403. [Full Text]  



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