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First published online 21 November 2006
doi: 10.1242/jcs.03294

Journal of Cell Science 119, 5047-5056 (2006)
Published by The Company of Biologists 2006
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Research Article

The Cdi/TESK1 kinase is required for Sevenless signaling and epithelial organization in the Drosophila eye

Marta Sesé1, Montserrat Corominas1, Hugo Stocker2, Tapio I. Heino3, Ernst Hafen2 and Florenci Serras1,*

1 Departament de Genètica, Facultat de Biologia, Universitat de Barcelona, Diagonal 645, 08028 Barcelona, Spain
2 Institute for Molecular Systems Biology, ETH Zürich, c/o Zoologisches Institut, Universität Zürich, Winterthurerstr. 190, 8057 Zürich, Switzerland
3 Institute of Biotechnology and Department of Biological and Environmental Sciences, University of Helsinki, FIN-00014 Helsinki, Finland

* Author for correspondence (e-mail: fserras{at}ub.edu)

Accepted 10 October 2006

How cellular behaviors such as cell-to-cell communication, epithelial organization and cell shape reorganization are coordinated during development is poorly understood. The developing Drosophila eye offers an ideal model system to study these processes. Localized actin polymerization is required to constrict the apical surface of epithelial cells of the eye imaginal disc to maintain the refined arrangement of the developing ommatidia. The identity of each photoreceptor cell within the epithelium is determined by cell-to-cell contacts involving signal transduction events. The R7 photoreceptor cell requires the activity of the Sevenless RTK to adopt a proper cell fate. We performed an EP screen for negative regulators of this inductive process, and we identified the serine/threonine kinase Center divider (cdi) as a suppressor of the phenotype caused by an activated Sevenless receptor. Cdi is homologous to the human testis-specific kinase 1 (TESK1), a member of the LIM kinases involved in cytoskeleton control through ADF/cofilin phosphorylation. We have analyzed the effects of gain- and loss-of-function of cdi and found alterations in actin organization and in the adherens junctions proteins DE-cadherin and ß-catenin, as well as in Sevenless apical localization. Interference with the function of the ADF/cofilin phosphatase Slingshot (ssh), which antagonizes Cdi, also results in a suppression of signaling triggered by the Sevenless RTK. Our results reveal a critical interplay between the localization of molecules involved in epithelial organization and signal transduction.

Key words: cdi, Sevenless, Photoreceptor, Eye, Actin




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