QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

First published online 31 October 2006
doi: 10.1242/jcs.03235

This Article Figures Only Full Text Full Text (PDF) Supplemental Material All Versions of this Article: jcs.03235v1 119/22/4770    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Cesario, J. M. Articles by McKim, K. S. Search for Related Content PubMed PubMed Citation Articles by Cesario, J. M. Articles by McKim, K. S.

Kinesin 6 family member Subito participates in mitotic spindle assembly and interacts with mitotic regulators

Waksman Institute and Department of Genetics, Rutgers, The State University of New Jersey, Piscataway, NJ 08854-8020, USA

^* Author for correspondence (e-mail: mckim{at}rci.rutgers.edu)

Accepted 30 August 2006

Drosophila Subito is a kinesin 6 family member and ortholog of mitotic kinesin-like protein (MKLP2) in mammalian cells. Based on the previously established requirement for Subito in meiotic spindle formation and for MKLP2 in cytokinesis, we investigated the function of Subito in mitosis. During metaphase, Subito localized to microtubules at the center of the mitotic spindle, probably interpolar microtubules that originate at the poles and overlap in antiparallel orientation. Consistent with this localization pattern, subito mutants improperly assembled microtubules at metaphase, causing activation of the spindle assembly checkpoint and lagging chromosomes at anaphase. These results are the first demonstration of a kinesin 6 family member with a function in mitotic spindle assembly, possibly involving the interpolar microtubules. However, the role of Subito during mitotic anaphase resembles other kinesin 6 family members. Subito localizes to the spindle midzone at anaphase and is required for the localization of Polo, Incenp and Aurora B. Genetic evidence suggested that the effects of subito mutants are attenuated as a result of redundant mechanisms for spindle assembly and cytokinesis. For example, subito double mutants with ncd, polo, Aurora B or Incenp mutations were synthetic lethal with severe defects in microtubule organization.

Key words: Mitosis, Microtubule, Drosophila, Central spindle, Kinesin-like protein, Checkpoint

This article has been cited by other articles:

				C. Wu, V. Singaram, and K. S. McKim mei-38 Is Required for Chromosome Segregation During Meiosis in Drosophila Females Genetics, September 1, 2008; 180(1): 61 - 72. [Abstract] [Full Text] [PDF]

				J. K. Jang, T. Rahman, V. S. Kober, J. Cesario, and K. S. McKim Misregulation of the Kinesin-like Protein Subito Induces Meiotic Spindle Formation in the Absence of Chromosomes and Centrosomes Genetics, September 1, 2007; 177(1): 267 - 280. [Abstract] [Full Text] [PDF]