spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online 12 September 2006
doi: 10.1242/jcs.03166

Journal of Cell Science 119, 3994-4001 (2006)
Published by The Company of Biologists 2006
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary Material
Right arrow All Versions of this Article:
jcs.03166v1
119/19/3994    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kuravi, K.
Right arrow Articles by van der Klei, I. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kuravi, K.
Right arrow Articles by van der Klei, I. J.

Research Article

Dynamin-related proteins Vps1p and Dnm1p control peroxisome abundance in Saccharomyces cerevisiae

Kasinath Kuravi1,*, Shirisha Nagotu1,*, Arjen M. Krikken1, Klaas Sjollema1, Markus Deckers2, Ralf Erdmann2, Marten Veenhuis1 and Ida J. van der Klei1,{ddagger}

1 Eukaryotic Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, PO Box 14, NL-9750 AA Haren, The Netherlands
2 Institut für Physiologische Chemie, Abt. Systembiochemie, Ruhr-Universität Bochum, 44801 Bochum, Germany

{ddagger} Author for correspondence (e-mail: i.j.van.der.klei{at}rug.nl)

Accepted 13 July 2006

Saccharomyces cerevisiae contains three dynamin-related-proteins, Vps1p, Dnm1p and Mgm1p. Previous data from glucose-grown VPS1 and DNM1 null mutants suggested that Vps1p, but not Dnm1p, plays a role in regulating peroxisome abundance. Here we show that deletion of DNM1 also results in reduction of peroxisome numbers. This was not observed in glucose-grown dnm1 cells, but was evident in cells grown in the presence of oleate. Similar observations were made in cells lacking Fis1p, a protein involved in Dnm1p function. Fluorescence microscopy of cells producing Dnm1-GFP or GFP-Fis1p demonstrated that both proteins had a dual localization on mitochondria and peroxisomes. Quantitative analysis revealed a greater reduction in peroxisome number in oleate-induced vps1 cells relative to dnm1 or fis1 cells. A significant fraction of oleate-induced vps1 cells still contained two or more peroxisomes. Conversely, almost all cells of a dnm1 vps1 double-deletion strain contained only one, enlarged peroxisome. This suggests that deletion of DNM1 reinforces the vps1 peroxisome phenotype. Time-lapse imaging indicated that during budding of dnm1 vps1 cells, the single peroxisome present in the mother cell formed long protrusions into the developing bud. This organelle divided at a very late stage of the budding process, possibly during cytokinesis.

Key words: Yeast, Peroxisome, Dynamin-related-proteins, Vps1p, Dnm1p, Saccharomyces cerevisiae




This article has been cited by other articles:


Home page
 Plant CellHome page
M. J. Lingard, S. K. Gidda, S. Bingham, S. J. Rothstein, R. T. Mullen, and R. N. Trelease
Arabidopsis PEROXIN11c-e, FISSION1b, and DYNAMIN-RELATED PROTEIN3A Cooperate in Cell Cycle-Associated Replication of Peroxisomes
PLANT CELL, June 1, 2008; 20(6): 1567 - 1585.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
S.-i. Arimura, M. Fujimoto, Y. Doniwa, N. Kadoya, M. Nakazono, W. Sakamoto, and N. Tsutsumi
Arabidopsis ELONGATED MITOCHONDRIA1 Is Required for Localization of DYNAMIN-RELATED PROTEIN3A to Mitochondrial Fission Sites
PLANT CELL, June 1, 2008; 20(6): 1555 - 1566.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
A. M. Motley, G. P. Ward, and E. H. Hettema
Dnm1p-dependent peroxisome fission requires Caf4p, Mdv1p and Fis1p
J. Cell Sci., May 15, 2008; 121(10): 1633 - 1640.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
F. Liu, S. K. Ng, Y. Lu, W. Low, J. Lai, and G. Jedd
Making two organelles from one: Woronin body biogenesis by peroxisomal protein sorting
J. Cell Biol., January 28, 2008; 180(2): 325 - 339.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. C. Wells, L. K. Picton, S. C. P. Williams, F. J. Tan, and R. B. Hill
Direct Binding of the Dynamin-like GTPase, Dnm1, to Mitochondrial Dynamics Protein Fis1 Is Negatively Regulated by the Fis1 N-terminal Arm
J. Biol. Chem., November 16, 2007; 282(46): 33769 - 33775.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
A. M. Motley and E. H. Hettema
Yeast peroxisomes multiply by growth and division
J. Cell Biol., July 24, 2007; 178(3): 399 - 410.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2006