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First published online 22 August 2006
doi: 10.1242/jcs.03148

Journal of Cell Science 119, 3845-3855 (2006)
Published by The Company of Biologists 2006
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Research Article

Arl1p is involved in transport of the GPI-anchored protein Gas1p from the late Golgi to the plasma membrane

Ya-Wen Liu, Szu-Wei Lee and Fang-Jen S. Lee*

Institute of Molecular Medicine, School of Medicine, National Taiwan University, and Department of Medical Research, National Taiwan University Hospital, Taipei 100, Taiwan

* Author for correspondence (e-mail: fangjen{at}ha.mc.ntu.edu.tw)

Accepted 21 June 2006

The molecular mechanisms involved in the transport of GPI-anchored proteins from the trans-Golgi network (TGN) to the cell periphery have not been established. Arl1p is a member of the Arf-like protein (Arl) subfamily of small GTPases and is localized in the late Golgi. Although Arl1p is implicated in regulation of Golgi structure and function, no endogenous cargo protein that is regulated by Arl1p has been identified in yeast. In this study, we demonstrate that Arl1p is involved in the anterograde transport from the Golgi to the cell surface of the glycosylphosphatidylinositol (GPI)-anchored plasma-membrane-resident protein Gas1p, but not the cell-wall-localized GPI-anchored proteins Crh1p, Crh2p and Cwp1p, or non-GPI-anchored plasma membrane-protein Gap1p. We also show that regulators of Arl1p (Sys1p, Arl3p and Gcs1p) and an effector (Imh1p) all participate in the transport of Gas1p. Thus, we infer that the signaling cascade Sys1p-Arl3p-Arl1p-Imh1p specifically participates in the transport of a GPI-anchored protein from the late Golgi to the plasma membrane.

Key words: ARF, GTPase, Golgi, GPI-anchored protein


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Arl1p drops GPI anchor at the membrane

JCS 2006 119: 1803. [Full Text]  



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P.-C. Tsai, S.-W. Lee, Y.-W. Liu, C.-W. Chu, K.-Y. Chen, J.-C. Ho, and F.-J. S. Lee
Afi1p Functions as an Arf3p Polarization-specific Docking Factor for Development of Polarity
J. Biol. Chem., June 13, 2008; 283(24): 16915 - 16927.
[Abstract] [Full Text] [PDF]


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