spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online 27 June 2006
doi: 10.1242/jcs.03030

Journal of Cell Science 119, 2995-3007 (2006)
Published by The Company of Biologists 2006
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary Material
Right arrow All Versions of this Article:
jcs.03030v1
119/14/2995    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Grati, M.
Right arrow Articles by Wenthold, R. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Grati, M.
Right arrow Articles by Wenthold, R. J.

Research Article

Molecular determinants for differential membrane trafficking of PMCA1 and PMCA2 in mammalian hair cells

M'hamed Grati1,*, Nisha Aggarwal1, Emanuel E. Strehler2 and Robert J. Wenthold1,{ddagger}

1 Laboratory of Neurochemistry, National Institute on Deafness and other Communication Disorders, National Institutes of Health, Bethesda, MD 20892, USA
2 Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, Rochester, MN 55905, USA

{ddagger} Author for correspondence (e-mail: wenthold{at}nidcd.nih.gov)

Accepted 2 May 2006

The plasma membrane Ca2+-ATPase-2 (PMCA2) is expressed in stereocilia of hair cells of the inner ear, whereas PMCA1 is expressed in the basolateral plasma membrane of hair cells. Both extrude excess Ca2+ from the cytosol. They are predicted to contain ten membrane-spanning segments, two large cytoplasmic loops as well as cytosolic N- and C-termini. Several isoform variants are generated for both PMCA1 and PMCA2 by alternative splicing, affecting their first cytosolic loop (A-site) and their C-terminal tail. To understand how these isoforms are differentially targeted in hair cells, we investigated their targeting regions and expression in hair cells. Our results show that a Leu-Ile motif in `b'-tail splice variants promotes PMCA1b and PMCA2b basolateral sorting in hair cells. Moreover, apical targeting of PMCA2 depends on the size of the A-site-spliced insert, suggesting that the conformation of the cytoplasmic loop plays a role in apical targeting.

Key words: Alternative splicing, Inner ear explant, Intracellular loop, Leucine-isoleucine signal, Stereocilia




This article has been cited by other articles:


Home page
 Mol. Biol. CellHome page
M. Carmosino, I. Gimenez, M. Caplan, and B. Forbush
Exon Loss Accounts for Differential Sorting of Na-K-Cl Cotransporters in Polarized Epithelial Cells
Mol. Biol. Cell, October 1, 2008; 19(10): 4341 - 4351.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
J. N. VanHouten, M. C. Neville, and J. J. Wysolmerski
The Calcium-Sensing Receptor Regulates Plasma Membrane Calcium Adenosine Triphosphatase Isoform 2 Activity in Mammary Epithelial Cells: A Mechanism for Calcium-Regulated Calcium Transport into Milk
Endocrinology, December 1, 2007; 148(12): 5943 - 5954.
[Abstract] [Full Text] [PDF]

Home page
 PhysiologyHome page
F. Mammano, M. Bortolozzi, S. Ortolano, and F. Anselmi
Ca2+ Signaling in the Inner Ear
Physiology, April 1, 2007; 22(2): 131 - 144.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2006