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First published online June 8, 2006
doi: 10.1242/10.1242/jcs.02967

Journal of Cell Science 119, 2486-2496 (2006)
Published by The Company of Biologists 2006
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Research Article

The Arabidopsis thaliana MND1 homologue plays a key role in meiotic homologous pairing, synapsis and recombination

C. Kerzendorfer1,2,*, J. Vignard3,*, A. Pedrosa-Harand1, T. Siwiec1, S. Akimcheva2, S. Jolivet3, R. Sablowski4, S. Armstrong5, D. Schweizer1,2, R. Mercier3,{ddagger} and P. Schlögelhofer1,{ddagger}

1 Department of Chromosome Biology, Max F. Perutz Laboratories, University of Vienna, A-1030 Vienna, Austria
2 Gregor Mendel Institute of Molecular Plant Biology, Austrian Academy of Sciences, A-1030 Vienna, Austria
3 Station de Génétique et d'Amélioration des Plantes, Institut National de la Recherche Agronomique, 78026 Versailles CEDEX, France
4 Department of Cell and Developmental Biology, John Innes Centre, Norwich, NR4 7UH, UK
5 School of Bioscience, University of Birmingham, Birmingham, B15 2TT, UK

{ddagger} Authors for correspondence (e-mail: rmercier{at}versailles.inra.fr; peter.schloegelhofer{at}univie.ac.at)

Accepted 1 March 2006

Mnd1 has recently been identified in yeast as a key player in meiotic recombination. Here we describe the identification and functional characterisation of the Arabidopsis homologue, AtMND1, which is essential for male and female meiosis and thus for plant fertility. Although axial elements are formed normally, sister chromatid cohesion is established and recombination initiation appears to be unaffected in mutant plants, chromosomes do not synapse. During meiotic progression, a mass of entangled chromosomes, interconnected by chromatin bridges, and severe chromosome fragmentation are observed. These defects depend on the presence of SPO11-1, a protein that initiates recombination by catalysing DNA double-strand break (DSB) formation. Furthermore, we demonstrate that the AtMND1 protein interacts with AHP2, the Arabidopsis protein closely related to budding yeast Hop2. These data demonstrate that AtMND1 plays a key role in homologous synapsis and in DSB repair during meiotic recombination.

Key words: Arabidopsis, Meiosis, Recombination, Synapsis




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