spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online 13 December 2005
doi: 10.1242/jcs.02704

Journal of Cell Science 119, 75-84 (2006)
Published by The Company of Biologists 2006
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary Material
Right arrow A correction has been published
Right arrow All Versions of this Article:
jcs.02704v1
119/1/75    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cotton, L.
Right arrow Articles by O'Bryan, M. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cotton, L.
Right arrow Articles by O'Bryan, M. K.

Research Article

FGFR-1 signaling is involved in spermiogenesis and sperm capacitation

Leanne Cotton1, Gerard M. Gibbs1, L. Gabriel Sanchez-Partida1, John R. Morrison1, David M. de Kretser1,2 and Moira K. O'Bryan1,2,*

1 Monash Institute of Medical Research, Monash University, Melbourne, Australia
2 The ARC Centre of Excellence in Biotechnology and Development, Monash University, Melbourne, Australia

* Author for correspondence (e-mail: Moira.obryan{at}med.monash.edu.au)

Accepted 21 September 2005

Cloning of the fibroblast growth factor receptor (FGFR) adaptor Snt-2 cDNA and the identification of FGFR-1 protein in association with sperm tails, suggested that FGFR-1 signaling was involved in either sperm tail development or function. This hypothesis was tested by the creation of transgenic mice that specifically expressed a dominant-negative variant of FGFR-1 in male haploid germ cells. Mating of transgenic mice showed a significant reduction in pups per litter compared with wild-type littermates. Further analysis demonstrated that this subfertility was driven by a combination of reduced daily sperm output and a severely compromised ability of those sperm that were produced to undergo capacitation prior to fertilization. An analysis of key signal transduction proteins indicated that FGFR-1 is functional on wild-type sperm and probably signals via the phosphatidylinositol 3-kinase pathway. FGFR-1 activation also resulted in the downstream suppression of mitogen activated protein kinase signaling. These data demonstrate the FGFR-1 is required for quantitatively and qualitatively normal spermatogenesis and has a key role in the regulation of the global tyrosine phosphorylation events associated with sperm capacitation.

Key words: FGFR-1, Sperm, Capacitation, Fertility, Spermatogenesis




This article has been cited by other articles:


Home page
 Am. J. Pathol.Home page
E. Gold, N. Jetly, M. K. O'Bryan, S. Meachem, D. Srinivasan, S. Behuria, L. G. Sanchez-Partida, T. Woodruff, S. Hedwards, H. Wang, et al.
Activin C Antagonizes Activin A in Vitro and Overexpression Leads to Pathologies in Vivo
Am. J. Pathol., January 1, 2009; 174(1): 184 - 195.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
F. Odet, C. Duan, W. D Willis, E. H Goulding, A. Kung, E. M Eddy, and E. Goldberg
Expression of the Gene for Mouse Lactate Dehydrogenase C (Ldhc) Is Required for Male Fertility
Biol Reprod, July 1, 2008; 79(1): 26 - 34.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
L. M. Cotton, M. K. O'Bryan, and B. T. Hinton
Cellular Signaling by Fibroblast Growth Factors (FGFs) and Their Receptors (FGFRs) in Male Reproduction
Endocr. Rev., April 1, 2008; 29(2): 193 - 216.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2006