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First published online 12 April 2005
doi: 10.1242/jcs.02311

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End4/Sla2 is involved in establishment of a new growth zone in Schizosaccharomyces pombe

¹ Cell Cycle Lab Cancer Research UK, 44 Lincoln's Inn Field, London, WC2A 3PX, UK
² Paul-Ehrlich-Institute, Paul-Ehrlich-Straße 51-59, 63225 Langen, Germany
³ Rockefeller University, 1230 York Avenue, New York, NY 10021, USA

^* Author for correspondence (e-mail: s.castagnetti{at}cancer.org.uk)

Accepted 7 February 2005

The rod-shaped Schizosaccharomyces pombe cell grows in a polarized fashion from opposing ends. Correct positioning of the growth zones is directed by the polarity marker Tea1 located at the cell ends where actin patches accumulate and cell growth takes place. We show that the S. pombe homologue of Saccharomyces cerevisiae SLA2, a protein involved in cortical actin organization and endocytosis, provides a link between the polarity marker and the growth machinery. In wild-type fission yeast cells, this homologue End4/Sla2 is enriched at cell ends during interphase and localizes to a medial ring at cell division, mirroring the actin localization pattern throughout the cell cycle. Proper localization relies on membrane trafficking and is independent of both the actin and microtubule cytoskeletons. End4/Sla2 is required for the establishment of new polarised growth zones, and deletion of its C-terminal talin-like domain prevents the establishment of a new growth zone after cell fission. We propose that End4/Sla2 acts downstream of the polarity marker Tea1 and is implicated in the recruitment of the actin cytoskeleton to bring about polarised cell growth.

Key words: End4/Sla2, fission yeast, Tea1, cell growth

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