spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online March 10, 2005
doi: 10.1242/10.1242/jcs.02284

Journal of Cell Science 118, 1105-1116 (2005)
Published by The Company of Biologists 2005
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow An erratum has been published
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rogers, G. C.
Right arrow Articles by Sharp, D. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rogers, G. C.
Right arrow Articles by Sharp, D. J.

Commentary

Spindle microtubules in flux

Gregory C. Rogers1,2,*, Stephen L. Rogers2 and David J. Sharp1

1 Department of Physiology and Biophysics, 223 Ullmann Building, Albert Einstein College of Medicine, Bronx, NY 10461, USA
2 Department of Biology, CB#3280, 241 Wilson Hall, University of North Carolina, Chapel Hill, NC 27599, USA

* Author for correspondence (e-mail: gcrogers{at}email.unc.edu)

Accurate and timely chromosome segregation is a task performed within meiotic and mitotic cells by a specialized force-generating structure – the spindle. This micromachine is constructed from numerous proteins, most notably the filamentous microtubules that form a structural framework for the spindle and also transmit forces through it. Poleward flux is an evolutionarily conserved mechanism used by spindle microtubules both to move chromosomes and to regulate spindle length. Recent studies have identified a microtubule-depolymerizing kinesin as a key force-generating component required for flux. On the basis of these findings, we propose a new model for flux powered by a microtubule-disassembly mechanism positioned at the spindle pole. In addition, we use the flux model to explain the results of spindle manipulation experiments to illustrate the importance of flux for proper chromosome positioning.

Key words: Mitosis, Spindle, Microtubule, Poleward flux, Kinesin




This article has been cited by other articles:


Home page
 J. Cell Biol.Home page
G. Yang, L. A. Cameron, P. S. Maddox, E. D. Salmon, and G. Danuser
Regional variation of microtubule flux reveals microtubule organization in the metaphase meiotic spindle
J. Cell Biol., August 25, 2008; 182(4): 631 - 639.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
I. A. Amaro, M. Costanzo, C. Boone, and T. C. Huffaker
The Saccharomyces cerevisiae Homolog of p24 Is Essential for Maintaining the Association of p150Glued With the Dynactin Complex
Genetics, February 1, 2008; 178(2): 703 - 709.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
N. P. Ferenz and P. Wadsworth
Prophase Microtubule Arrays Undergo Flux-like Behavior in Mammalian Cells
Mol. Biol. Cell, October 1, 2007; 18(10): 3993 - 4002.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. Liu and J. N. Onuchic
A driving and coupling "Pac-Man" mechanism for chromosome poleward translocation in anaphase A
PNAS, December 5, 2006; 103(49): 18432 - 18437.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
C. A. Moores and R. A. Milligan
Lucky 13 - microtubule depolymerisation by kinesin-13 motors.
J. Cell Sci., October 1, 2006; 119(Pt 19): 3905 - 3913.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
A. L. Pereira, A. J. Pereira, A. R.R. Maia, K. Drabek, C. L. Sayas, P. J. Hergert, M. Lince-Faria, I. Matos, C. Duque, T. Stepanova, et al.
Mammalian CLASP1 and CLASP2 Cooperate to Ensure Mitotic Fidelity by Regulating Spindle and Kinetochore Function
Mol. Biol. Cell, October 1, 2006; 17(10): 4526 - 4542.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
P. Dhonukshe, N. Vischer, and T. W. J. Gadella Jr
Contribution of microtubule growth polarity and flux to spindle assembly and functioning in plant cells.
J. Cell Sci., August 1, 2006; 119(Pt 15): 3193 - 3205.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
J. E. Laycock, M. S. Savoian, and D. M. Glover
Antagonistic activities of Klp10A and Orbit regulate spindle length, bipolarity and function in vivo
J. Cell Sci., June 1, 2006; 119(11): 2354 - 2361.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
L. A. Cameron, G. Yang, D. Cimini, J. C. Canman, O. Kisurina-Evgenieva, A. Khodjakov, G. Danuser, and E.D. Salmon
Kinesin 5-independent poleward flux of kinetochore microtubules in PtK1 cells
J. Cell Biol., April 24, 2006; 173(2): 173 - 179.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Manna, D. Thrower, H. P. Miller, P. Curmi, and L. Wilson
Stathmin Strongly Increases the Minus End Catastrophe Frequency and Induces Rapid Treadmilling of Bovine Brain Microtubules at Steady State in Vitro
J. Biol. Chem., January 27, 2006; 281(4): 2071 - 2078.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
G. Goshima, F. Nedelec, and R. D. Vale
Mechanisms for focusing mitotic spindle poles by minus end-directed motor proteins
J. Cell Biol., October 24, 2005; 171(2): 229 - 240.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2005