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First published online 29 November 2005
doi: 10.1242/jcs.02687

Journal of Cell Science 118, 5731-5742 (2005)
Published by The Company of Biologists 2005
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Research Article

Ace2p contributes to fission yeast septin ring assembly by regulating mid2+ expression

Claudia S. Petit, Sapna Mehta, Rachel H. Roberts and Kathleen L. Gould*

Howard Hughes Medical Institute, and Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN 37232, USA

* Author for correspondence (e-mail: kathy.gould{at}vanderbilt.edu)

Accepted 12 September 2005

The fission yeast Schizosaccharomyces pombe divides through constriction of an actomyosin-based contractile ring followed by formation and degradation of a medial septum. Formation of an organized septin ring is also important for the completion of S. pombe cell division and this event relies on the production of Mid2p. mid2+ mRNA and protein accumulate in mitosis. Recent microarray analyses identified mid2+ as a target of the Ace2p transcription factor, and ace2+ as a target of the Sep1p transcription factor. In this study, we find that Mid2p production is controlled by Ace2p functioning downstream of Sep1p. Consequently, both Sep1p and Ace2p are required for septin ring assembly and genetic analyses indicate that septin rings function in parallel with other Ace2p targets to achieve efficient cell division. Conversely, forced overproduction of Sep1p or Ace2p prevents septin ring disassembly. We find that Ace2p levels peak during anaphase and Ace2p is post-translationally modified by phosphorylation and ubiquitylation. Ace2p localizes symmetrically to dividing nuclei and functions independently of the septation initiation network.

Key words: Schizosaccharomyces pombe, Cytokinesis, Ace2p, Mid2p, Septins


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H.-J. Yoon, A. Feoktistova, J.-S. Chen, J. L. Jennings, A. J. Link, and K. L. Gould
Role of Hcn1 and Its Phosphorylation in Fission Yeast Anaphase-promoting Complex/Cyclosome Function
J. Biol. Chem., October 27, 2006; 281(43): 32284 - 32293.
[Abstract] [Full Text] [PDF]


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