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First published online 11 October 2005
doi: 10.1242/jcs.02613

Journal of Cell Science 118, 4995-5003 (2005)
Published by The Company of Biologists 2005
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Research Article

Interactions between Coilin and PIASy partially link Cajal bodies to PML bodies

Jun Sun1, Hongzhi Xu2, S. H. Subramony1 and Michael D. Hebert2,*

1 Department of Neurology, The University of Mississippi Medical Center, Jackson, MS 39216-4505, USA
2 Department of Biochemistry, The University of Mississippi Medical Center, Jackson, MS 39216-4505, USA

* Author for correspondence (e-mail: mhebert{at}biochem.umsmed.edu)

Accepted 3 August 2005

The eukaryotic nucleus contains a variety of dynamic structures, yet studies into the functional relationship of one type of subnuclear domain to another have been limited. For example, PML bodies and Cajal bodies associate, but the functional consequence of this association and the mediating factors are unknown. Here we report that an associated PML body/Cajal body can co-localize to an snRNA gene locus, with the Cajal body invariably situated between the PML body and the snRNA locus. Binding studies demonstrate that coilin (a Cajal body protein) directly interacts with PIASy (a PML body protein). Cell biological experiments using coilin and PIASy knockout cell lines demonstrate that interactions between coilin and PIASy account in part for the observed association of Cajal bodies with PML bodies. When the PIASy interaction region on coilin is deleted, the frequency of the association between Cajal bodies and PML bodies is reduced. These studies provide another example of coilin's role in the functional organization of the nucleus.

Key words: PIASy, SUMO, snRNPs, U snRNA gene loci, Nuclear organization


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