QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

First published online 27 September 2005
doi: 10.1242/jcs.02585

This Article Figures Only Full Text Full Text (PDF) Supplementary Material All Versions of this Article: jcs.02585v1 118/20/4655    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in JCS Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wargo, M. J. Articles by Smith, E. F. Search for Related Content PubMed PubMed Citation Articles by Wargo, M. J. Articles by Smith, E. F.

Calmodulin and PF6 are components of a complex that localizes to the C1 microtubule of the flagellar central apparatus

Dartmouth College, Department of Biological Sciences, 301 Gilman Hall, Hanover, NH 03755, USA

^* Author for correspondence (e-mail: elizabeth.f.smith{at}dartmouth.edu)

Accepted 14 July 2005

Studies of flagellar motility in Chlamydomonas mutants lacking specific central apparatus components have supported the hypothesis that the inherent asymmetry of this structure provides important spatial cues for asymmetric regulation of dynein activity. These studies have also suggested that specific projections associated with the C1 and C2 central tubules make unique contributions to modulating motility; yet, we still do not know the identities of most polypeptides associated with the central tubules. To identify components of the C1a projection, we took an immunoprecipitation approach using antibodies generated against PF6. The pf6 mutant lacks the C1a projection and possesses flagella that only twitch; calcium-induced modulation of dynein activity on specific doublet microtubules is also defective in pf6 axonemes. Our antibodies specifically precipitated five polypeptides in addition to PF6. Using mass spectrometry, we determined the amino acid identities of these five polypeptides. Most notably, the PF6-containing complex includes calmodulin. Using antibodies generated against each precipitated polypeptide, we confirmed that these polypeptides comprise a single complex with PF6, and we identified specific binding partners for each member of the complex. The finding of a calmodulin-containing complex as an asymmetrically assembled component of the central apparatus implicates the central apparatus in calcium modulation of flagellar waveform.

Key words: Flagella, Axoneme, Calmodulin, Dynein, Chlamydomonas

Related articles in JCS:

Flagella make waves

JCS 2005 118: 2001. [Full Text]  

This article has been cited by other articles:

				M. J. Schneider, M. Ulland, and R. D. Sloboda A Protein Methylation Pathway in Chlamydomonas Flagella Is Active during Flagellar Resorption Mol. Biol. Cell, October 1, 2008; 19(10): 4319 - 4327. [Abstract] [Full Text] [PDF]

				C. Yang, H. A. Owen, and P. Yang Dimeric heat shock protein 40 binds radial spokes for generating coupled power strokes and recovery strokes of 9 + 2 flagella J. Cell Biol., January 28, 2008; 180(2): 403 - 415. [Abstract] [Full Text] [PDF]

				E. E. Dymek and E. F. Smith A conserved CaM- and radial spoke associated complex mediates regulation of flagellar dynein activity J. Cell Biol., November 5, 2007; 179(3): 515 - 526. [Abstract] [Full Text] [PDF]

				X. Shu, J. Huang, Y. Dong, J. Choi, A. Langenbacher, and J.-N. Chen Na,K-ATPase {alpha}2 and Ncx4a regulate zebrafish left-right patterning Development, May 15, 2007; 134(10): 1921 - 1930. [Abstract] [Full Text] [PDF]

				K.-F. Lechtreck and G. B. Witman Chlamydomonas reinhardtii hydin is a central pair protein required for flagellar motility J. Cell Biol., February 12, 2007; 176(4): 473 - 482. [Abstract] [Full Text] [PDF]

				H. Ueno, Y. Iwataki, and O. Numata Homologues of Radial Spoke Head Proteins Interact with Ca2+/Calmodulin in Tetrahymena Cilia J. Biochem., October 1, 2006; 140(4): 525 - 533. [Abstract] [Full Text] [PDF]

				E. E. Dymek, D. Goduti, T. Kramer, and E. F. Smith A kinesin-like calmodulin-binding protein in Chlamydomonas: evidence for a role in cell division and flagellar functions J. Cell Sci., August 1, 2006; 119(15): 3107 - 3116. [Abstract] [Full Text] [PDF]