|
|
|
|
|
||
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
First published online 26 April 2005
doi: 10.1242/jcs.02329
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Research Article |
Department of Internal Medicine, Washington University School of Medicine, St Louis, MO 63110, USA
* Author for correspondence (e-mail: laing{at}id.wustl.edu)
Accepted 15 February 2005
ZO-1 is the major connexin-interacting protein in ROS 17/2.8 (ROS) osteoblastic cells. We examined the role of ZO-1 in Cx43-mediated gap junction formation and function in ROS cells that expressed the connexin-interacting fragment of ZO-1 (ROS/ZO-1dn) cells. Expression of this ZO-17-444 fusion protein in ROS cells disrupted the Cx43/ZO-1 interaction and decreased dye transfer by 85%, although Cx43 was retained on the plasma membrane as assessed by surface biotinylation. Fractionation of lysates derived from ROS/ZO-1dn cells on a 5-30% sucrose flotation gradient showed that 40% of the Cx43 floated into these sucrose gradients, whereas none of the Cx43 in ROS cell lysates entered the gradients, suggesting that more Cx43 is associated with lipid rafts in the transfected ROS cells than in lysates derived from untransfected ROS cells. In contrast to the ROS/ZO-1dn cells, ROS cells that over-expressed ZO-1 protein (ROS/ZO-1myc cells) exhibited increased gap junctional permeability and appositional membrane staining for Cx43. These data demonstrate that ZO-1 regulates Cx43-mediated gap junctional communication in osteoblastic cells and alters the membrane localization of Cx43. They suggest that ZO-1-mediated delivery of Cx43 from a lipid raft domain to gap junctional plaques may be an important regulatory step in gap junction formation.
Key words: Connexin, Gap junction, ZO-1, Lipid rafts
This article has been cited by other articles:
|
|
 |
|
  J. Gilleron, C. Fiorini, D. Carette, C. Avondet, M. M. Falk, D. Segretain, and G. Pointis Molecular reorganization of Cx43, Zo-1 and Src complexes during the endocytosis of gap junction plaques in response to a non-genomic carcinogen J. Cell Sci., December 15, 2008; 121(24): 4069 - 4078. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Derangeon, N. Bourmeyster, I. Plaisance, C. Pinet-Charvet, Q. Chen, F. Duthe, M. R. Popoff, D. Sarrouilhe, and J.-C. Herve RhoA GTPase and F-actin Dynamically Regulate the Permeability of Cx43-made Channels in Rat Cardiac Myocytes J. Biol. Chem., November 7, 2008; 283(45): 30754 - 30765. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Li, N. Kamasawa, C. Ciolofan, C. O. Olson, S. Lu, K. G. V. Davidson, T. Yasumura, R. Shigemoto, J. E. Rash, and J. I. Nagy Connexin45-Containing Neuronal Gap Junctions in Rodent Retina Also Contain Connexin36 in Both Apposing Hemiplaques, Forming Bihomotypic Gap Junctions, with Scaffolding Contributed by Zonula Occludens-1 J. Neurosci., September 24, 2008; 28(39): 9769 - 9789. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. E. Flores, X. Li, M. V. L. Bennett, J. I. Nagy, and A. E. Pereda Interaction between connexin35 and zonula occludens-1 and its potential role in the regulation of electrical synapses PNAS, August 26, 2008; 105(34): 12545 - 12550. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Colomer, L. A. Olivos Ore, N. Coutry, M.-N. Mathieu, S. Arthaud, P. Fontanaud, I. Iankova, F. Macari, E. Thouennon, L. Yon, et al. Functional Remodeling of Gap Junction-Mediated Electrical Communication between Adrenal Chromaffin Cells in Stressed Rats J. Neurosci., June 25, 2008; 28(26): 6616 - 6626. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Xu, P. J. Kausalya, D. C. Y. Phua, S. M. Ali, Z. Hossain, and W. Hunziker Early Embryonic Lethality of Mice Lacking ZO-2, but Not ZO-3, Reveals Critical and Nonredundant Roles for Individual Zonula Occludens Proteins in Mammalian Development Mol. Cell. Biol., March 1, 2008; 28(5): 1669 - 1678. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Langlois, K. N. Cowan, Q. Shao, B. J. Cowan, and D. W. Laird Caveolin-1 and -2 Interact with Connexin43 and Regulate Gap Junctional Intercellular Communication in Keratinocytes Mol. Biol. Cell, March 1, 2008; 19(3): 912 - 928. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. F. Bruce, S. Rothery, E. Dupont, and N. J. Severs Gap junction remodelling in human heart failure is associated with increased interaction of connexin43 with ZO-1 Cardiovasc Res, March 1, 2008; 77(4): 757 - 765. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. van Zeijl, B. Ponsioen, B. N.G. Giepmans, A. Ariaens, F. R. Postma, P. Varnai, T. Balla, N. Divecha, K. Jalink, and W. H. Moolenaar Regulation of connexin43 gap junctional communication by phosphatidylinositol 4,5-bisphosphate J. Cell Biol., June 21, 2007; 177(5): 881 - 891. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. D'hondt, S. P. Srinivas, J. Vereecke, and B. Himpens Adenosine Opposes Thrombin-Induced Inhibition of Intercellular Calcium Wave in Corneal Endothelial Cells Invest. Ophthalmol. Vis. Sci., April 1, 2007; 48(4): 1518 - 1527. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. D'hondt, R. Ponsaerts, S. P. Srinivas, J. Vereecke, and B. Himpens Thrombin Inhibits Intercellular Calcium Wave Propagation in Corneal Endothelial Cells by Modulation of Hemichannels and Gap Junctions Invest. Ophthalmol. Vis. Sci., January 1, 2007; 48(1): 120 - 133. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Ichimiya and T. Kojima Cellular Networks of Human Thymic Medullary Stromas Coordinated by p53-Related Transcription Factors J. Histochem. Cytochem., November 1, 2006; 54(11): 1277 - 1289. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Maguy, T. E. Hebert, and S. Nattel Involvement of lipid rafts and caveolae in cardiac ion channel function Cardiovasc Res, March 1, 2006; 69(4): 798 - 807. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. W. Hunter, R. J. Barker, C. Zhu, and R. G. Gourdie Zonula Occludens-1 Alters Connexin43 Gap Junction Size and Organization by Influencing Channel Accretion Mol. Biol. Cell, December 1, 2005; 16(12): 5686 - 5698. [Abstract] [Full Text] [PDF]
|
|
